Novel insights into differences in the whey proteome of porcine and human colostrum and mature milk.

Many mothers are unable to exclusively breastfeed for several reasons. Other mammalian milks should be explored as human milk supplements. Pigs and humans are physiologically similar. Therefore, we hypothesized that the whey protein composition and functions of porcine and human milk are similar. This study aimed to compare and analyze the whey proteomes of porcine and human milk during lactation. Totally, 3390, 3275, 3587, and 3463 whey proteins were identified in porcine colostrum, porcine mature milk, human colostrum, and human mature milk, respectively. Complement components and serotransferrin, which are related to the immune system process were highly expressed in porcine milk. Porcine and human whey proteins were enriched in similar Gene Ontology annotations and Kyoto Encyclopedia of Genes and Genomes pathways. These results revealed that the biological functions of porcine and human whey proteins were similar, which contributes to the development of porcine whey proteins.