Bäck O, Egelrud T
Br J Dermatol. 1985 May;112(5):539-45. doi: 10.1111/j.1365-2133.1985.tb15261.x.
A mouse model for assessment of the anti-inflammatory effect of topical glucocorticoids is described. Mice, sensitized to picryl chloride, had both ears painted with the sensitizer followed 2 hours later by the application of the topical steroid to one ear and the corresponding vehicle to the other. The contact sensitivity reaction, measured by swelling of the ear, was recorded 24 hours later. Dilutions of the steroid formulations inhibited the ear swelling in a manner related to dose-response. Suppression of the contact sensitivity reaction of the vehicle-treated ear as well was regarded as a systemic effect of the glucocorticoid. There seems to be a good correlation between the efficacy of the topical steroids assessed in this mouse model and the vasoconstrictor test on intact human skin.
本文描述了一种用于评估局部糖皮质激素抗炎作用的小鼠模型。对苦味酸氯致敏的小鼠双耳均涂抹致敏剂，2小时后，在一只耳朵上涂抹局部类固醇，另一只耳朵涂抹相应的赋形剂。24小时后记录通过耳朵肿胀测量的接触敏感性反应。类固醇制剂的稀释液以剂量反应相关的方式抑制耳朵肿胀。赋形剂处理耳朵的接触敏感性反应受到抑制也被视为糖皮质激素的全身作用。在该小鼠模型中评估的局部类固醇的疗效与完整人体皮肤上的血管收缩试验之间似乎存在良好的相关性。
