Roupe G, Ridell B
Acta Derm Venereol. 1979;59(3):191-5.
In the present work, the contact hypersensitivity skin test reaction to picryl chloride in CBA mice was examined. The test was performed by applying the contact allergen to the ear skin and making a series of histological analyses up to 24 hours after challenge. An increment in ear thickness, measured with an engineer's micrometer 24 hours after challenge, was obvious in a group of sensitized mice when compared with a nonsensitized control group, and the difference was found to be highly significant. One hour after challenge, mononuclear cells appeared in the dermis, increasing in numbers during the following 12 hours. At this time, neutrophil granulocytes were the dominant cells in the infiltrate and remained so up to 24 hours after challenge. On the basis of the experiments performed here we conclude that measuring of the ear swelling with a micrometer 24 hours after challenge is a useful and reliable test of contact sensitivity in the mouse.
在本研究中，检测了CBA小鼠对氯化苦基的接触性超敏皮肤试验反应。通过将接触性变应原涂抹于耳皮肤，并在激发后长达24小时进行一系列组织学分析来进行该试验。与未致敏对照组相比，在激发后24小时用工程师用测微计测量，一组致敏小鼠的耳厚度增加明显，且发现差异具有高度显著性。激发后1小时，单核细胞出现在真皮中，在随后的12小时内数量增加。此时，中性粒细胞是浸润中的主要细胞，并且在激发后长达24小时一直如此。基于此处进行的实验，我们得出结论，在激发后24小时用测微计测量耳肿胀是小鼠接触敏感性的一种有用且可靠的检测方法。
