Sakamoto H, Ooshima A
Br J Haematol. 1985 May;60(1):173-81. doi: 10.1111/j.1365-2141.1985.tb07398.x.
Phagocytosis by human neutrophils of opsonized sheep erythrocytes with rabbit immunoglobulin M and human complement (IgM-EACh) and those with rabbit immunoglobulin G (IgG-EA) increased 2-3 times of control neutrophils after treatment with released products from activated platelets (PRPr). The factors in PRPr which augmented IgM-EACh phagocytosis were suggested to be adenosine diphosphate (ADP) and triphosphate (ATP) by experiments using apyrase treated PRPr and by direct exposures of neutrophils with these nucleotides. The presence of two other stimulators for phagocytosis of IgG-EA were suggested. One was a macromolecular substance of molecular weight greater than 100 000 and the other was a substance of molecular weight less than 500. The latter may be a prostaglandin because the activity was easily removed by acid-extraction with ethylacetate from the water phase, and because prior incubation of platelets with 10 micrograms per ml indomethacin deprived PRPr of its low molecular weight activity. Exposure of neutrophils to thromboxane B2, prostaglandin F2 alpha and prostaglandin E2 at the concentration of 10(-6)-10(-7) mg/ml actually increased the phagocytosis of IgG-EA. The activity in the macromolecular fraction was not affected by prior incubation of platelets with indomethacin.
用来自活化血小板的释放产物（PRPr）处理后，人中性粒细胞对经兔免疫球蛋白M和人补体调理的绵羊红细胞（IgM-EACh）以及经兔免疫球蛋白G调理的绵羊红细胞（IgG-EA）的吞噬作用比对照中性粒细胞增加了2至3倍。使用经腺苷三磷酸双磷酸酶处理的PRPr进行的实验以及使中性粒细胞直接接触这些核苷酸的实验表明，PRPr中增强IgM-EACh吞噬作用的因子是二磷酸腺苷（ADP）和三磷酸腺苷（ATP）。有人提出存在另外两种刺激IgG-EA吞噬作用的物质。一种是分子量大于100000的大分子物质，另一种是分子量小于500的物质。后者可能是一种前列腺素，因为该活性很容易通过用乙酸乙酯从水相中进行酸提取而去除，并且因为预先将血小板与每毫升10微克消炎痛一起孵育会使PRPr失去其低分子量活性。使中性粒细胞暴露于浓度为10(-6)-10(-7)毫克/毫升的血栓素B2、前列腺素F2α和前列腺素E2实际上会增加IgG-EA的吞噬作用。大分子部分的活性不受预先将血小板与消炎痛一起孵育的影响。
