Henson P M
Immunology. 1969 Jan;16(1):107-21.
Human, rabbit and guinea-pig neutrophils and rabbit and guinea-pig macrophages were shown to adhere to sheep erythrocytes sensitized with isologous IgG antibody (EAIgG). IgM antibody, however, was less active and use of rabbit IgM antibody to fix complement from different species allowed the examination of neutrophil, macrophage and platelet adherence to alexinated erythrocytes (EAIgMC′). Complement from twelve mammalian species induced the adherence of isologous neutrophils. On the other hand, platelets showed some variability. Ox, goat, sheep and pig platelets, like those from man and the baboon, did not adhere to EAIgMC′, although adherence of platelets from the rabbit, guinea-pig, rat, mouse, horse and cat was observed. C′3 was the complement component implicated in these reactions. Treatment of the leucocytes with trypsin prevented the adherence to EAIgMC′ but not to EAIgG. Chelation of free magnesium with EDTA inhibited the adherence of rabbit neutrophils, but not of human neutrophils, to EAIgMC′. EDTA had no effect on the adherence to EAIgG. Rabbit and guinea-pig neutrophils exhibited some species specificity towards the antibody and complement which would induce their adherence. This was not shown by human neutrophils or by rabbit and guinea-pig macrophages. The adherence of particles to phagocytes comprises the first stage of phagocytosis and the role of antibody and complement in this process is discussed.
已证实人类、兔和豚鼠的中性粒细胞以及兔和豚鼠的巨噬细胞能黏附于用同种IgG抗体致敏的绵羊红细胞（EAIgG）。然而，IgM抗体的活性较低，使用兔IgM抗体来固定不同物种的补体，可用于检测中性粒细胞、巨噬细胞和血小板对经补体处理的红细胞（EAIgMC′）的黏附。来自12种哺乳动物的补体可诱导同种中性粒细胞的黏附。另一方面，血小板表现出一定的变异性。牛、山羊、绵羊和猪的血小板，与人及狒狒的血小板一样，不黏附于EAIgMC′，不过观察到兔、豚鼠、大鼠、小鼠、马和猫的血小板有黏附现象。C′3是参与这些反应的补体成分。用胰蛋白酶处理白细胞可阻止其对EAIgMC′的黏附，但不影响对EAIgG的黏附。用EDTA螯合游离镁可抑制兔中性粒细胞对EAIgMC′的黏附，但不影响人类中性粒细胞对EAIgMC′的黏附。EDTA对黏附于EAIgG无影响。兔和豚鼠的中性粒细胞对能诱导其黏附的抗体和补体表现出一定的物种特异性。人类中性粒细胞以及兔和豚鼠的巨噬细胞则未表现出这种特异性。颗粒对吞噬细胞的黏附是吞噬作用的第一阶段，本文讨论了抗体和补体在这一过程中的作用。
