Muralidharan F N, Muralidharan V B
Biochim Biophys Acta. 1985 Jun 14;835(1):36-40.
The enzymatic conversion of phytol to phytanic acid has been demonstrated in vitro in rat liver. Subcellular fractionation indicated that the mitochondrial fraction possessed the highest activity. Substantial activity was also present in the microsomal fraction. A new bromination-thin-layer chromatography procedure was developed to separate the phytol-dihydrophytol mixture and this procedure was applied to identify, characterize and quantitate the metabolites of phytol-phytanate conversion, i.e., phytanic acid, phytenic acid and dihydrophytol. Phytanic and phytenic acids were formed in the ratio 100:7.4. The conversion of phytol to phytenic acid was in the range 2-3%. No dihydrophytol was detected over boiled, acidified, or no-enzyme controls. The presence of phytenic acid and the absence of dihydrophytol in the incubation mixture confirm the previous in vivo studies and suggest that phytenic acid may be an intermediate in phytol-phytanate conversion.
植醇向植烷酸的酶促转化已在大鼠肝脏的体外实验中得到证实。亚细胞分级分离表明，线粒体部分活性最高。微粒体部分也存在大量活性。开发了一种新的溴化 - 薄层色谱法来分离植醇 - 二氢植醇混合物，并将该方法用于鉴定、表征和定量植醇 - 植烷酸转化的代谢产物，即植烷酸、植烯酸和二氢植醇。植烷酸和植烯酸的形成比例为100:7.4。植醇向植烯酸的转化率在2 - 3%范围内。在煮沸、酸化或无酶对照中未检测到二氢植醇。孵育混合物中植烯酸的存在和二氢植醇的缺失证实了先前的体内研究，并表明植烯酸可能是植醇 - 植烷酸转化的中间体。
