Vahabi Maedeh, Rostamian Abdolrahman, Mirsharif Ensie Sadat, Latifi Keyvan, Iranparast Sara, Ghazanfari Tooba
Immunoregulation Research Center, Shahed University, Tehran, Iran.
Rheumatology Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2025 Feb 13;24(1):21-30. doi: 10.18502/ijaai.v24i1.18017.
COVID-19 is capable of undermining self-tolerance in a host's immune system and triggering autoimmunity by hyper-activating the innate and adaptive immune systems, which has not investigated in Iranian society until now. In the innate immune system neutrophils are the predominant immune cells that protect the human body against invaders. Here, we sought to explore 2 important variables related to neutrophil: DNA complexes with myeloperoxidase (MPO-DNA) as a reliable indicator of neutrophil extracellular traps (NETs) by MPO-DNA complex evaluation using a sandwich ELISA and the underlying role of IL-8 in (NETs) formation during COVID-19 infection. According to our results, in 103 COVID-19 patients, neutrophil-to-lymphocyte ratio (NLR) was significantly higher in ICU patients (14.62±11.81) compared to non-ICU patients (3.16±3.33). Elevated IL-8 levels were observed in COVID-19 patients, particularly in ICU patients. MPO-DNA levels, indicating NETosis, were significantly higher in COVID-19 patients and strongly correlated with neutrophil counts (r=0.472). Thus, our studies suggest that neutrophils count, IL-8, and MPO-DNA can be used as powerful biomarkers in diagnosing COVID-19 severity. patients with severe COVID-19 infection are prone to heart disease because most of them develop excessive NET formation. Additionally, In COVID-19 patients, a higher MPO-DNA level may increase the risk of developing heart disease too.
新型冠状病毒肺炎能够破坏宿主免疫系统中的自身耐受性，并通过过度激活先天性和适应性免疫系统引发自身免疫，而伊朗社会至今尚未对此进行研究。在先天性免疫系统中，中性粒细胞是保护人体抵御入侵者的主要免疫细胞。在此，我们试图探讨与中性粒细胞相关的两个重要变量：作为中性粒细胞胞外诱捕网（NETs）可靠指标的髓过氧化物酶与DNA复合物（MPO-DNA），通过夹心酶联免疫吸附测定法评估MPO-DNA复合物，以及白细胞介素-8在新型冠状病毒肺炎感染期间NETs形成中的潜在作用。根据我们的研究结果，在103例新型冠状病毒肺炎患者中，重症监护病房（ICU）患者的中性粒细胞与淋巴细胞比值（NLR）（14.62±11.81）显著高于非ICU患者（3.16±3.33）。新型冠状病毒肺炎患者，尤其是ICU患者，白细胞介素-8水平升高。表明NETosis的MPO-DNA水平在新型冠状病毒肺炎患者中显著升高，且与中性粒细胞计数密切相关（r=0.472）。因此，我们的研究表明，中性粒细胞计数、白细胞介素-8和MPO-DNA可作为诊断新型冠状病毒肺炎严重程度的有力生物标志物。新型冠状病毒肺炎严重感染患者易患心脏病，因为他们中的大多数人会形成过多的NETs。此外，在新型冠状病毒肺炎患者中，较高的MPO-DNA水平也可能增加患心脏病的风险。
