一种基于牛精子细胞活力的快速定量方法，用于生物材料的体外毒性测试。

A rapid quantitative method based on motility of bull sperm cells for in vitro toxicity testing of biomaterials.

作者信息

Petzoldt R, Wolf H, Reefschläger J, Karge M, Berger G

出版信息

Biomaterials. 1985 Mar;6(2):105-9. doi: 10.1016/0142-9612(85)90072-9.

Abstract

A quantitative method for in vitro toxicity testing of biomaterials, based on measurements of time-dependent changes of bull sperm motility, is described. In comparison to the haemolytic and toxic effects of biomaterials such as polyurethanes, poly(vinyl chloride) tubes and bioglass ceramics on erythrocytes and the proliferation rate of human embryonic lung fibroblasts, this method is shown to be more sensitive. The simplicity, rapidity and reproducibility of the test under application of genetically identical cells are advantages that make it suitable for screening large numbers of samples. The quantification of test results allows inter-laboratory comparison.

摘要

本文描述了一种基于测量公牛精子活力随时间变化的生物材料体外毒性测试定量方法。与聚氨酯、聚氯乙烯管和生物玻璃陶瓷等生物材料对红细胞的溶血和毒性作用以及人胚肺成纤维细胞的增殖率相比，该方法显示出更高的灵敏度。在使用基因相同的细胞进行测试时，该测试具有简单、快速和可重复性等优点，使其适用于大量样品的筛选。测试结果的量化允许进行实验室间比较。