Li Yuqi, Tian Xue, Zhang Lina, Lin Jing, Wang Qian, Gu Lingwen, Li Hong, Yu Bing, Wang Ziyi, Chi Menghui, Zhao Guiqiu
Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Exp Eye Res. 2025 May;254:110323. doi: 10.1016/j.exer.2025.110323. Epub 2025 Mar 5.
Fungal keratitis (FK) is a severe vision-threatening eye disease. The fungal invasiveness and excessive inflammatory response contribute to corneal tissue damage. Rutin (RT) possesses anti-inflammatory, antimicrobial, antioxidant, and improved wound-healing characteristics. This study aimed to evaluate antifungal, anti-inflammatory, and therapeutic effects of RT in FK. The results showed that RT exerted antifungal effects by inhibiting fungal growth, altering hyphal morphology, destroying biofilm, and disrupting fungal cellular structures. RT exhibited anti-inflammatory benefits by suppressing the Dectin-1/p-Syk pathway, activating the Nrf2/HO-1 pathway, and decreasing the expression of inflammatory factors in vivo and in vitro. RT demonstrated therapeutic effects by reducing clinical scores, fungal load, macrophage recruitment, and neutrophil activity. In conclusion, RT exhibited anti-inflammatory, antifungal, and therapeutic effects in Aspergillus fumigatus keratitis, and has the potential to become a novel therapeutic strategy for FK.
真菌性角膜炎（FK）是一种严重威胁视力的眼部疾病。真菌的侵袭性和过度的炎症反应会导致角膜组织损伤。芦丁（RT）具有抗炎、抗菌、抗氧化和促进伤口愈合的特性。本研究旨在评估RT对FK的抗真菌、抗炎和治疗效果。结果表明，RT通过抑制真菌生长、改变菌丝形态、破坏生物膜和破坏真菌细胞结构发挥抗真菌作用。RT通过抑制Dectin-1/p-Syk途径、激活Nrf2/HO-1途径以及在体内和体外降低炎症因子的表达而表现出抗炎作用。RT通过降低临床评分、真菌负荷、巨噬细胞募集和中性粒细胞活性而显示出治疗效果。总之，RT在烟曲霉角膜炎中表现出抗炎、抗真菌和治疗作用，并且有潜力成为FK的一种新型治疗策略。
