Guan Zhengzhe, Wang Mengjiao, Ma Jiayi, Mu Mengyuan, Li Xinyu, Sun Peimeng, Zhang Mengyuan, Zhang Yuanyuan, Song Luyang, Wen Caiyi, Zhao Ying
College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
Arch Virol. 2025 Mar 8;170(4):69. doi: 10.1007/s00705-025-06263-z.
In this study, we isolated a novel mycovirus from Lasiodiplodia pseudotheobromae strain YY-1, which we named "Lasiodiplodia pseudotheobromae mycovirus 1" (LpMyV1). The complete genome of LpMyV1 is 2877 bp in length and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a putative protein of 316 amino acids with a molecular weight of 34.1 kDa that shares similarity with the coat proteins of several mycoviruses. ORF2 encodes a protein of 561 amino acids with a molecular weight of 63.2 kDa that contains a conserved RNA-dependent RNA polymerase (RdRp) domain. BLASTp results showed that the RdRp of LpMyV1 shares the highest similarity with those of non-segmented dsRNA viruses. A multiple sequence alignment and phylogenetic analysis indicated that LpMyV1 is a new member of the proposed genus "Unirnavirus". This is the first report of a mycovirus from L. pseudotheobromae.
在本研究中，我们从可可毛色二孢菌菌株YY-1中分离出一种新型真菌病毒，我们将其命名为“可可毛色二孢菌真菌病毒1”（LpMyV1）。LpMyV1的完整基因组长度为2877 bp，包含两个不重叠的开放阅读框（ORF1和ORF2）。ORF1编码一个推定的由316个氨基酸组成的蛋白质，分子量为34.1 kDa，与几种真菌病毒的外壳蛋白具有相似性。ORF2编码一个由561个氨基酸组成的蛋白质，分子量为63.2 kDa，包含一个保守的RNA依赖性RNA聚合酶（RdRp）结构域。BLASTp结果表明，LpMyV1的RdRp与非分段双链RNA病毒的RdRp具有最高的相似性。多序列比对和系统发育分析表明，LpMyV1是拟议的“单分病毒属”的一个新成员。这是关于可可毛色二孢菌中真菌病毒的首次报道。
