Tan Wenye, Tian Jingfei, Zhao Wenfeng, Wei Jianxin, Xu Yibo, Zhou Shixu, Wei Zihan, Shen Zejun, Wu Minghang, Shang Lianguang, Li Rongbai, Wang Yongfei, Qin Baoxiang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Plant Mol Biol. 2025 Mar 8;115(2):40. doi: 10.1007/s11103-025-01574-w.
The fatty acyl ACP thioesterases, catalyzing the final step of fatty acid synthesis in the plastid, regulate various critical processes in plants, including seed oil accumulation, seed development, plant growth, and drought tolerance. However, their roles in male fertility have seldom been demonstrated. In this study, the function of a newly FAT, Impaired Pollen Fertility 1 (IPF1) in male fertility was investigated. IPF1 expressed prominently in microspores and tapetum. IPF1 specifically located in the endoplasmic reticulum. IPF1 knock-out mutants produced by the CRISPR/Cas9 system displayed significant reduction in seed-setting rate compared to WT. The decreased seed-setting rate in the ipf1 mutants was found to be attributed to the defects of pollen viability, not the female gamete fertility. The aborted pollen in the ipf1 mutants showed impaired pollen wall formation and diminished lipid deposition. Consistently, the expression levels of six genes critical to pollen wall formation and lipid metabolism (GPAT3, OsC6, DPW2, OsPKS1, OsPKS2, and OsSTRL2) were significantly decreased in the ipf1 mutant. Taken together, these results demonstrate that IPF1 regulates rice pollen fertility through the modulation of lipid synthesis.
脂肪酰基-ACP硫酯酶催化质体中脂肪酸合成的最后一步，调控植物中的各种关键过程，包括种子油积累、种子发育、植物生长和耐旱性。然而，它们在雄性育性中的作用鲜有报道。在本研究中，对一种新的FAT，即花粉育性受损1（IPF1）在雄性育性中的功能进行了研究。IPF1在小孢子和绒毡层中显著表达。IPF1特异性定位于内质网。通过CRISPR/Cas9系统产生的IPF1敲除突变体与野生型相比结实率显著降低。发现ipf1突变体中结实率降低是由于花粉活力缺陷，而非雌配子育性。ipf1突变体中败育的花粉显示出花粉壁形成受损和脂质沉积减少。一致地，在ipf1突变体中，六个对花粉壁形成和脂质代谢至关重要的基因（GPAT3、OsC6、DPW2、OsPKS1、OsPKS2和OsSTRL2）的表达水平显著降低。综上所述，这些结果表明IPF1通过调节脂质合成来调控水稻花粉育性。
