Xu Yibo, Zhou Shixu, Tian Jingfei, Zhao Wenfeng, Wei Jianxin, He Juan, Tan Wenye, Shang Lianguang, He Xinhua, Li Rongbai, Wang Yongfei, Qin Baoxiang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Theor Appl Genet. 2024 Dec 3;138(1):1. doi: 10.1007/s00122-024-04786-8.
DDP1, encoding a β-Ketoacyl-CoA Synthase, regulates rice anther dehiscence and pollen fertility by affecting the deposition of lipid on anther epidermis and pollen wall. Anther dehiscence and pollen fertility are crucial for male fertility in rice. Here, we studied the function of Defective in Dehiscence and Pollen1 (DDP1), a novel member of the KCS family in rice, in regulating anther dehiscence and pollen fertility. DDP1 encodes an endoplasmic reticulum (ER)-localized protein and is ubiquitously expressed in various organs, predominately in the microspores and tapetum. The ddp1 mutant exhibited partial male sterility attributed to defective anther dehiscence and pollen fertility, which was notably distinct from those observed in Arabidopsis thaliana and rice mutants associated with lipid metabolism. Mutations of DDP1 altered the content and composition of wax on anther epidermis and pollen wall, causing abnormalities in their morphology. Moreover, genes implicated in lipid metabolism, pollen development, and anther dehiscence exhibited significantly altered expression levels in the ddp1 mutant. These findings indicate that DDP1 controls anther dehiscence and pollen fertility to ensure normal male development by modulating lipid homeostasis in the tapetum, thereby enhancing our understanding of the mechanisms underlying rice anther dehiscence and pollen fertility.
DDP1编码一种β-酮脂酰辅酶A合酶，通过影响脂质在花药表皮和花粉壁上的沉积来调节水稻花药开裂和花粉育性。花药开裂和花粉育性对水稻雄性育性至关重要。在此，我们研究了水稻KCS家族的一个新成员——开裂和花粉缺陷1（DDP1）在调节花药开裂和花粉育性中的功能。DDP1编码一种定位于内质网（ER）的蛋白质，在各种器官中普遍表达，主要在小孢子和绒毡层中表达。ddp1突变体表现出部分雄性不育，这归因于花药开裂和花粉育性缺陷，这与在拟南芥和与脂质代谢相关的水稻突变体中观察到的情况明显不同。DDP1的突变改变了花药表皮和花粉壁上蜡质的含量和组成，导致其形态异常。此外，与脂质代谢、花粉发育和花药开裂相关的基因在ddp1突变体中的表达水平发生了显著变化。这些发现表明，DDP1通过调节绒毡层中的脂质稳态来控制花药开裂和花粉育性，以确保正常的雄性发育，从而加深了我们对水稻花药开裂和花粉育性潜在机制的理解。
