十六烷基三甲基溴化铵（CETAB）修饰的纳米蒙脱石对黄曲霉毒素B1相关毒性及产甲烷作用的体外功效

In vitro efficacy of cetyltrimethylammonium bromide (CETAB)-modified nano-montmorillonite against aflatoxin B1 associated toxicity and methanogenesis.

作者信息

Soltan Yosra A, Morsy Amr S, Hashem Nesrein M, Elazab Mahmoud A I, Sultan Mohamed A, El-Nile Amr, El Lail Gomaa Abo, El-Desoky Nagwa, Hosny Nourhan S, Mahdy Ahmed M, Hafez Elsayed E, Sallam Sobhy M A

机构信息

Animal and Fish Production Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.

Livestock Research Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt.

出版信息

BMC Vet Res. 2025 Mar 8;21(1):155. doi: 10.1186/s12917-025-04546-w.

DOI:10.1186/s12917-025-04546-w

PMID:40057750

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11889834/

Abstract

BACKGROUND

Modified nano-montmorillonite is gaining attention as a feed additive for its benefits on ruminal fermentation. Chemical and mechanical methods were used to modify montmorillonite. Cetyltrimethylammonium bromide (CETAB) was utilized for chemical modification, while grounding was carried out to achieve the desired nanoscale particle size, resulting in the formation of the nanoscale powder known as MNM. Impacts of MNM supplementation on a basal diet, either contaminated with aflatoxin B1 (AFB1) or not at a level of 20 ppb were tested. Treatments included control (no supplements), a diet with 5 g per kilogram of dry matter (DM) of natural montmorillonite (NM), and diets with MNM at two doses, 0.5 (low) and 1 (high) grams per kilogram DM.

RESULTS

The MNM showed better physicochemical traits than NM clay, including narrower particle size range, higher cation exchange capacity (CEC), greater specific surface area (SSA), and more functional groups. A significant linear decreasing effect (P < 0.05) of MNM addition on methane (CH) production was observed by the increasing level of the MNM clay. The control diet contaminated with AFB1 resulted in lower fiber degradability than the other treatments (P < 0.05). No variations were observed in ruminal protozoal counts by both clay supplementations, although there was a noticeable trend (P = 0.08) towards reduced protozoal populations due to AFB1 contamination. AFB1-contaminated diets showed indications of reduced (P < 0.05) levels of total volatile fatty acids (VFA), and concentrations of butyrate and propionate (P < 0.05), alongside shifts towards elevated (P = 0.006) acetate levels, while the low dose of MNM exhibited higher (P < 0.01) propionate concentrations than the other treatments.

CONCLUSION

These findings underscored the anti-methanogenic properties and the favorable impacts of MNM in mitigating the adverse impacts of AFB1on ruminal fermentation and nutrient degradability.

摘要

背景

改性纳米蒙脱石作为一种饲料添加剂，因其对瘤胃发酵的有益作用而受到关注。采用化学和机械方法对蒙脱石进行改性。十六烷基三甲基溴化铵（CETAB）用于化学改性，同时进行研磨以达到所需的纳米级粒径，从而形成称为MNM的纳米级粉末。测试了添加MNM对基础日粮的影响，基础日粮分别被黄曲霉毒素B1（AFB1）污染或未被污染，污染水平为20 ppb。处理包括对照组（不添加）、每千克干物质（DM）含5克天然蒙脱石（NM）的日粮，以及每千克DM含0.5克（低剂量）和1克（高剂量）MNM的两种剂量的日粮。

结果

MNM表现出比NM粘土更好的物理化学特性，包括更窄的粒径范围、更高的阳离子交换容量（CEC）、更大的比表面积（SSA）和更多的官能团。随着MNM粘土添加量的增加，观察到MNM添加对甲烷（CH）产生有显著的线性降低效应（P < 0.05）。被AFB1污染的对照日粮导致纤维降解率低于其他处理（P < 0.05）。两种粘土添加处理均未观察到瘤胃原虫数量的变化，尽管由于AFB1污染，原虫数量有明显的减少趋势（P = 0.08）。被AFB1污染的日粮显示总挥发性脂肪酸（VFA）水平降低（P < 0.05），丁酸和丙酸浓度降低（P < 0.05），同时乙酸水平升高（P = 0.006），而低剂量的MNM表现出比其他处理更高的丙酸浓度（P < 0.01）。