磷酸化壳聚糖与纳米尺寸的替硝唑：增强修复性玻璃离子水门汀的强度、抗生物膜作用及生物相容性。

Phosphorylated chitosan and nano-sized TMP: Enhancing strength, antibiofilm action, and biocompatibility of restorative glass ionomer cements.

作者信息

Piati Gabrielle Cristina, Silva Danielly Beatriz Gonçalves, Delbem Alberto Carlos Botazzo, Martorano Antônio Secco, Raucci Larissa Moreira Spinola de Castro, de Oliveira Paulo Tambasco, Zucolotto Valtencir, Dias Bruna Juliana Moreira, Brighenti Fernanda Lourenção, de Oliveira Analú Barros, Moraes João Carlos Silos, de Camargo Emerson Rodrigues, Vidilli André Luiz, Danelon Marcelle

机构信息

Department of Pediatric Clinics, School of Dentistry of Ribeirão Preto, São Paulo University, Avenida do Cafe, s/no, Ribeirão Preto, SP, Brazil.

School of Dentistry, University of Ribeirão Preto - UNAERP, Ribeirão Preto, SP CEP 14096-900, Brazil.

出版信息

J Dent. 2025 May;156:105675. doi: 10.1016/j.jdent.2025.105675. Epub 2025 Mar 7.

DOI:10.1016/j.jdent.2025.105675

PMID:40058484

Abstract

OBJECTIVES

Evaluated the effect of phosphorylated chitosan (ChiPh) and nano-sized sodium trimetaphosphate (TMPnano) incorporation into resin modified glass ionomer cement (RMGIC) on mechanical, fluoride release, antimicrobial and cytotoxic properties.

METHODS

RMGIC was combined with ChiPh (0.25 %/0.5 %) and/or TMPnano (14 %). The diametral compressive/tensile strength (DCS/TS), surface hardness (SH) and degree of conversion (%DC) were determined. For fluoride (F) release, samples were immersed in DE/RE solutions. Antimicrobial/antibiofilm activity was evaluated by agar diffusion test/biofilm metabolism. Cytotoxicity on MDPC-23 odontoblast cell lines was evaluated.

RESULTS

TS and DCS (24 h), all the groups were similar (p > 0.05). After 7 days, RMGIC-14 %TMPnano-0.25 %ChiPh showed the best results for TS/DCS (p < 0.05). The SH was lower and similar for the RMGIC and RMGI-14 %TMPnano-0.5 %ChiPh groups (p > 0.05) after 24 h; after 7 days, all groups containing TMPnano and/or ChiPh showed higher SH when compared to RMGIC, except for RMGIC-0.5 % ChiPh. The %DC was similar for all groups (p = 0.172). There was a gradual release of F for all groups during the 15 days; in the cumulative analysis, after 15 days, the RMGIC-14 %TMPnano-0.25 %ChiPh group showed the highest value (p < 0.05). Viability against S. mutans was observed for the RMGIC-14 %TMPnano-0.25 %ChiPh group (p < 0.05). After 24 h, the RMGIC-0.25 %ChiPh and RMGIC-14 %TMPnano-0.25 %ChiPh groups showed the lowest cytotoxic effect (p = 0.371); at 48 h/72 h, only the RMGIC-14 %TMPnano-0.25 %ChiPh group showed similar cytocompatibility.

CONCLUSION

The addition of ChiPh (0.25 %) and TMPnano (14 %) improved the RMGIC's mechanical, antimicrobial/antibiofilm and cytotoxic properties.

CLINICAL RELEVANCE

ChiPh and TMPnano into RMGIC could be a promising restorative material for application in patients with active dental caries.

摘要

目的

评估将磷酸化壳聚糖（ChiPh）和纳米级三聚偏磷酸钠（TMPnano）掺入树脂改性玻璃离子水门汀（RMGIC）对其机械性能、氟释放性能、抗菌性能和细胞毒性的影响。

方法

将RMGIC与ChiPh（0.25%/0.5%）和/或TMPnano（14%）混合。测定其径向抗压/抗拉强度（DCS/TS）、表面硬度（SH）和转化率（%DC）。对于氟（F）释放，将样品浸入去离子水/再矿化溶液中。通过琼脂扩散试验/生物膜代谢评估抗菌/抗生物膜活性。评估对MDPC-23成牙本质细胞系的细胞毒性。

结果

TS和DCS（24小时），所有组相似（p>0.05）。7天后，RMGIC-14%TMPnano-0.25%ChiPh组的TS/DCS表现最佳（p<0.05）。24小时后，RMGIC组和RMGI-14%TMPnano-0.5%ChiPh组的SH较低且相似（p>0.05）；7天后，与RMGIC相比，所有含TMPnano和/或ChiPh的组SH更高，但RMGIC-0.5%ChiPh组除外。所有组的%DC相似（p=0.172）。在15天内所有组的F均呈逐渐释放；在累积分析中，15天后，RMGIC-14%TMPnano-0.25%ChiPh组的值最高（p<0.05）。观察到RMGIC-14%TMPnano-0.25%ChiPh组对变形链球菌有生存能力（p<0.05）。24小时后，RMGIC-0.25%ChiPh组和RMGIC-14%TMPnano-0.25%ChiPh组的细胞毒性最低（p=0.371）；在48小时/72小时时，只有RMGIC-14%TMPnano-0.25%ChiPh组表现出相似的细胞相容性。