Itkis Daniel G, Brooks F Phil, Davis Hunter C, Hotter Raphael, Wong-Campos J David, Qi Yitong, Jia Bill Z, Howell Madeleine, Xiong Marley, Hayward Rebecca Frank, Lee Byung Hun, Wang Yangdong, Perelman Rebecca T, Cohen Adam E
Department of Chemistry and Chemical Biology, Harvard University, Cambridge MA 02138.
bioRxiv. 2025 Feb 27:2025.02.22.639658. doi: 10.1101/2025.02.22.639658.
Bidirectional microscopy (BDM) combines simultaneous targeted optical perturbation and imaging of biophysical or biochemical signals (e.g. membrane voltage, Ca, or signaling molecules). A core challenge in BDM is precise spatial and temporal alignment of stimulation, imaging, and other experimental parameters. Here we present Luminos, an open-source MATLAB library for modular and precisely synchronized control of BDM experiments. The system supports hardware-triggered synchronization across stimulation, recording, and imaging channels with microsecond accuracy. Source code and documentation for Luminos are available online at https://www.luminosmicroscopy.com and https://github.com/adamcohenlab/luminos-microscopy. This library will facilitate development of bidirectional microscopy methods across the biological sciences.
双向显微镜（BDM）结合了同时进行的靶向光学扰动以及生物物理或生化信号（例如膜电压、钙离子或信号分子）的成像。BDM的一个核心挑战是刺激、成像和其他实验参数的精确空间和时间对齐。在此，我们展示了Luminos，这是一个用于模块化且精确同步控制BDM实验的开源MATLAB库。该系统支持跨刺激、记录和成像通道的硬件触发同步，精度可达微秒级。Luminos的源代码和文档可在https://www.luminosmicroscopy.com和https://github.com/adamcohenlab/luminos-microscopy在线获取。该库将促进生物科学领域双向显微镜方法的开发。
