Park Pojeong, Wong-Campos J David, Itkis Daniel G, Lee Byung Hun, Qi Yitong, Davis Hunter C, Antin Benjamin, Pasarkar Amol, Grimm Jonathan B, Plutkis Sarah E, Holland Katie L, Paninski Liam, Lavis Luke D, Cohen Adam E
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Department of Brain Sciences, DGIST, Daegu, Republic of Korea.
Nat Commun. 2025 Feb 4;16(1):1333. doi: 10.1038/s41467-025-55819-9.
Dendrites on neurons support electrical excitations, but the computational significance of these events is not well understood. We developed molecular, optical, and computational tools for all-optical electrophysiology in dendrites. We mapped sub-millisecond voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons under diverse optogenetic and synaptic stimulus patterns, in acute brain slices. Our data show history-dependent spike back-propagation in distal dendrites, driven by locally generated Na spikes (dSpikes). Dendritic depolarization created a transient window for dSpike propagation, opened by A-type K channel inactivation, and closed by slow Na inactivation. Collisions of dSpikes with synaptic inputs triggered calcium channel and N-methyl-D-aspartate receptor (NMDAR)-dependent dendritic plateau potentials and accompanying complex spikes at the soma. This hierarchical ion channel network acts as a spike-rate accelerometer, providing an intuitive picture connecting dendritic biophysics to associative plasticity rules.
神经元上的树突支持电兴奋，但这些事件的计算意义尚未得到很好的理解。我们开发了用于树突全光学电生理学的分子、光学和计算工具。在急性脑切片中，我们在不同的光遗传学和突触刺激模式下，绘制了CA1锥体神经元整个树突树中的亚毫秒级电压动态。我们的数据显示，由局部产生的钠尖峰（dSpikes）驱动，远端树突中存在依赖历史的尖峰反向传播。树突去极化为dSpike传播创造了一个瞬态窗口，该窗口由A型钾通道失活打开，并由缓慢的钠失活关闭。dSpikes与突触输入的碰撞触发了钙通道和N-甲基-D-天冬氨酸受体（NMDAR）依赖的树突平台电位以及伴随的胞体复杂尖峰。这种分层离子通道网络充当尖峰速率加速度计，提供了将树突生物物理学与联想可塑性规则联系起来的直观图景。
