Yeh Jiunn-Tyng, Hsiao Chen-Yun, Lee Hsuan-Wo, Kuo Chang-Fu
Novascope Diagnostics Inc, 2F.-9, No. 38, Taiyuan St., Zhubei 302082, Hsinchu County, Taiwan.
Center for Artificial Intelligence in Medicine, Chang Gung Memorial Hospital, Research Building third Floor AI Core Lab, No. 15 Wenhua first Rd., Taoyuan 333, Guishan Dist., Taiwan.
ACS Omega. 2025 Feb 17;10(8):8433-8439. doi: 10.1021/acsomega.4c10449. eCollection 2025 Mar 4.
This study investigates the performance and stability of extended-gate field-effect transistor (EGFET) pH sensors with three different reference electrode configurations: conventional external Ag/AgCl, external gold (Au), and integrated Au electrodes. The goal is to identify an optimal reference electrode configuration for enhancing the portability and manufacturability of EGFET pH sensors, which are crucial in medical, agricultural, and environmental applications. Results showed that the integrated Au electrode exhibits superior stability and comparable sensitivity to those of Ag/AgCl and external Au electrodes. The integrated Au electrode configuration achieved a 96 mV/pH sensitivity with 97% linearity across a pH range of 4 to 10. Stability tests revealed that the integrated Au electrode had the least drift under different pH conditions compared with the other two configurations, suggesting improved reliability. These findings highlight the integrated Au electrode's potential as a viable alternative to conventional reference electrodes in EGFET pH sensors, offering enhanced performance and practicality for point-of-care testing and other applications.
本研究调查了具有三种不同参比电极配置的扩展栅场效应晶体管（EGFET）pH传感器的性能和稳定性：传统外部Ag/AgCl电极、外部金（Au）电极和集成Au电极。目标是确定一种最佳参比电极配置，以提高EGFET pH传感器的便携性和可制造性，这在医疗、农业和环境应用中至关重要。结果表明，集成Au电极表现出优于Ag/AgCl电极和外部Au电极的稳定性，且灵敏度相当。集成Au电极配置在4至10的pH范围内实现了96 mV/pH的灵敏度和97%的线性度。稳定性测试表明，与其他两种配置相比，集成Au电极在不同pH条件下的漂移最小，这表明其可靠性更高。这些发现突出了集成Au电极在EGFET pH传感器中作为传统参比电极可行替代方案的潜力，为即时检测和其他应用提供了更高的性能和实用性。
