Wang Zhi-Ru, Kang Wen-Ting, Yu Rui-Li
Department of Pathology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China.
Clinical Operations Department, Jiangsu Hengrui Pharmaceuticals Co., Ltd, Nanjing, Jiangsu, China.
Front Oncol. 2025 Feb 21;15:1530924. doi: 10.3389/fonc.2025.1530924. eCollection 2025.
As a deubiquitinase, ubiquitin-specific protease 7 (USP7) plays a vital role in diverse cancers, nevertheless, its role in gastric cancer (GC), which is the fifth leading cause of death in diverse cancers worldwide, has rarely been reported.
To gain a comprehensive understanding about USP7 in the progression of GC, 287 paired GC tissues were collected and analyzed. ShRNA and small molecular inhibitor were used to investigate the impact of USP7 on cell proliferation. Additionally, xenograft proliferation was used to explore the effect of USP7 on tumor growth in animals.
We found that USP7 overexpression in GC tissues was correlated with several parameters of clinicopathology and poor disease-free survival rate. Meanwhile, the abrogation of USP7 suppressed GC cell proliferation by stabilizing p53 and promoting cell cycle arrest both in vitro and in vivo.
These results indicate that USP7 may serve as a biomarker for predicting the outcomes of patients with GC. Therefore, USP7 could be a promising therapeutic target for GC treatment.
作为一种去泛素化酶，泛素特异性蛋白酶7（USP7）在多种癌症中发挥着至关重要的作用，然而，其在胃癌（GC）中的作用鲜有报道，而胃癌是全球各种癌症中导致死亡的第五大主要原因。
为全面了解USP7在胃癌进展中的作用，收集并分析了287对胃癌组织。使用短发夹RNA（shRNA）和小分子抑制剂来研究USP7对细胞增殖的影响。此外，利用异种移植瘤增殖来探究USP7对动物肿瘤生长的作用。
我们发现胃癌组织中USP7的过表达与临床病理学的几个参数以及无病生存率低相关。同时，USP7的缺失通过稳定p53和促进细胞周期停滞在体外和体内均抑制了胃癌细胞的增殖。
这些结果表明USP7可能作为预测胃癌患者预后的生物标志物。因此，USP7可能是胃癌治疗中一个有前景的治疗靶点。
