Zhang Haoyu, Yang Huixuan, Du Sha, Ren Jiaxin, Qiao Guofeng, Ren Jie
College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, 030801, China.
College of Animal Science, Shanxi Agricultural University, Jinzhong, 030801, China.
Urolithiasis. 2025 Mar 10;53(1):50. doi: 10.1007/s00240-025-01726-z.
Kidney stones are a common urological disease. Although there are many ways to treat them, their high recurrence rate remains unresolved. Research has demonstrated that Lysimachia christinae Hance influences kidney stone development; however, the exact mechanism remains unclear. In this experiment, we investigated the protective effect of Lysimachia christinae Hance extract, rutin, on renal injury and its mechanism of action in a mouse model of glyoxalate-induced renal calculi. A kidney stone model was established in Balb/c mice by continuous intraperitoneal injection of glyoxalate (80 mg/kg) for 6 days. Simultaneous gavage of 30, and 60 mg/kg of rutin was administered for 6 days. Samples were collected for determination of index coefficients. Mouse kidney tissue was collected for RNA-seq, and segments of mouse colon were analyzed with 16 S RNA sequencing. We found that rutin significantly reduced renal calcium oxalate deposition and renal tissue injury in the glyoxylate-induced kidney stone model of mice. Rutin also significantly inhibited calcium oxalate deposition-induced apoptosis. In addition, rutin attenuates oxidative stress damage and inhibits the expression of signaling pathways associated with inflammation. 16 S RNA sequencing revealed that rutin regulated intestinal flora composition, significantly increasing the relative abundance of short-chain fatty acid-producing flora and promoting short-chain fatty acid production. In summary, rutin ameliorated renal tubular damage and apoptosis caused by renal stone deposition and reduced oxidative stress. It also regulates the intestinal flora, increases the enrichment of intestinal probiotics, and promotes the production of short-chain fatty acids, thereby inhibiting the formation of kidney stones.
肾结石是一种常见的泌尿系统疾病。尽管有多种治疗方法，但它们的高复发率仍未得到解决。研究表明，金钱草会影响肾结石的发展；然而，确切机制仍不清楚。在本实验中，我们研究了金钱草提取物芦丁对乙醛酸诱导的肾结石小鼠模型肾损伤的保护作用及其作用机制。通过连续6天腹腔注射乙醛酸（80mg/kg）在Balb/c小鼠中建立肾结石模型。同时灌胃给予30mg/kg和60mg/kg的芦丁，持续6天。收集样本以测定指标系数。收集小鼠肾脏组织进行RNA测序，并对小鼠结肠段进行16S RNA测序。我们发现芦丁显著减少了乙醛酸诱导的小鼠肾结石模型中的肾草酸钙沉积和肾组织损伤。芦丁还显著抑制了草酸钙沉积诱导的细胞凋亡。此外，芦丁减轻氧化应激损伤并抑制与炎症相关的信号通路的表达。16S RNA测序显示芦丁调节肠道菌群组成，显著增加产生短链脂肪酸的菌群的相对丰度并促进短链脂肪酸的产生。总之，芦丁改善了肾结石沉积引起的肾小管损伤和细胞凋亡，并降低了氧化应激。它还调节肠道菌群，增加肠道益生菌的富集，并促进短链脂肪酸的产生，从而抑制肾结石的形成。
