Zhang Lili, Cui Wenxing, Tang Dandan, Lin Haohong, Dong Weiliang, Qian Xiujuan, Jiang Min
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800, P. R. China.
State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211800, P. R. China.
Curr Microbiol. 2025 Mar 10;82(4):184. doi: 10.1007/s00284-025-04162-9.
Scheffersomyces segobiensis DSM27193 has been identified as a potential host for Palmitoleic acid (POA)-rich lipids production. Our previous study has found that S. segobiensis DSM27193 possesses a broad substrate spectrum, excellent xylose fermentation capability, rapid growth, high biomass, and an abundance of intracellular acetyl-CoA. These characteristics make S. segobiensis DSM27193 an ideal chassis for metabolic engineering and the synthesis of various chemicals. However, the genetic coding features of this strain remain unclear. Analysis of the internal transcribed spacer (ITS) region suggested that S. segobiensis DSM27193 is closely related to Pichia stipitis (renamed Scheffersomyces stipitis), both of which exhibit CTG coding characteristic. In this study, we utilized software tools, such as CodonW, CUPS, and others to analyze the codon preference of the S. segobiensis DSM27193 genome. Additionally, we validated the reliability of the codon table through the expression of green fluorescent protein (GFP). The analysis results serve as a theoretical foundation for enhancing the expression of exogenous genes in S. segobiensis DSM27193.
戈壁舍弗酵母DSM27193已被确定为生产富含棕榈油酸（POA）脂质的潜在宿主。我们之前的研究发现，戈壁舍弗酵母DSM27193具有广泛的底物谱、出色的木糖发酵能力、快速生长、高生物量以及丰富的细胞内乙酰辅酶A。这些特性使戈壁舍弗酵母DSM27193成为代谢工程和各种化学品合成的理想底盘。然而，该菌株的遗传编码特征仍不清楚。对内部转录间隔区（ITS）区域的分析表明，戈壁舍弗酵母DSM27193与树干毕赤酵母（现更名为树干舍弗酵母）密切相关，二者均表现出CTG编码特征。在本研究中，我们利用CodonW、CUPS等软件工具分析了戈壁舍弗酵母DSM27193基因组的密码子偏好性。此外，我们通过绿色荧光蛋白（GFP）的表达验证了密码子表的可靠性。分析结果为提高外源基因在戈壁舍弗酵母DSM27193中的表达提供了理论基础。
