Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Engler-Bunte-Ring 1, Karlsruhe, 76131, Germany.
AMB Express. 2014 Mar 18;4:24. doi: 10.1186/s13568-014-0024-0. eCollection 2014.
The yeast strains Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis were isolated from soil samples and identified as oleaginous yeast strains beneficial for the establishment of microbial production processes for sustainable lipid production suitable for several industrial applications. When cultured in bioreactors with glucose as the sole carbon source C. podzolicus yielded 31.8% lipid per dry biomass at 20°C, while T. porosum yielded 34.1% at 25°C and P. segobiensis 24.6% at 25°C. These amounts correspond to lipid concentrations of 17.97 g/L, 17.02 g/L and 12.7 g/L and volumetric productivities of 0.09 g/Lh, 0.1 g/Lh and 0.07 g/Lh, respectively. During the culture of C. podzolicus 30 g/l gluconic acid was detected as by-product in the culture broth and 12 g/L gluconic acid in T. porosum culture. The production of gluconic acid was eliminated for both strains when glucose was substituted by xylose as the carbon source. Using xylose lipid yields were 11.1 g/L and 13.9 g/L, corresponding to 26.8% and 33.4% lipid per dry biomass and a volumetric productivity of 0.07 g/Lh and 0.09 g/Lh, for C. podzolicus and T. porosum respectively. The fatty acid profile analysis showed that oleic acid was the main component (39.6 to 59.4%) in all three strains and could be applicable for biodiesel production. Palmitic acid (18.4 to 21.1%) and linolenic acid (7.5 to 18.7%) are valuable for cosmetic applications. P. segobiensis had a considerable amount of palmitoleic acid (16% content) and may be suitable for medical applications.
从土壤样本中分离出的酵母菌株Cryptococcus podzolicus、Trichosporon porosum 和 Pichia segobiensis 被鉴定为产油酵母菌株，有利于建立适合多种工业应用的可持续脂质生产的微生物生产工艺。当以葡萄糖作为唯一碳源在生物反应器中培养时，C. podzolicus 在 20°C 下产生 31.8%的干生物量脂质，而 T. porosum 在 25°C 下产生 34.1%，P. segobiensis 在 25°C 下产生 24.6%。这些数值分别对应于 17.97 g/L、17.02 g/L 和 12.7 g/L 的脂质浓度以及 0.09 g/L/h、0.1 g/L/h 和 0.07 g/L/h 的容积产率。在 C. podzolicus 的培养过程中，检测到 30 g/L 的葡萄糖酸作为发酵液中的副产物，而在 T. porosum 的培养中检测到 12 g/L 的葡萄糖酸。当葡萄糖被木糖替代作为碳源时，两种菌株的葡萄糖酸产量都被消除。使用木糖，脂质产量分别为 11.1 g/L 和 13.9 g/L，对应于 C. podzolicus 和 T. porosum 的干生物量分别为 26.8%和 33.4%的脂质和 0.07 g/L/h 和 0.09 g/L/h 的容积产率。脂肪酸谱分析表明，所有三种菌株的主要成分都是油酸（39.6%至 59.4%），可用于生物柴油生产。棕榈酸（18.4%至 21.1%）和亚麻酸（7.5%至 18.7%）对于化妆品应用具有很高的价值。P. segobiensis 含有相当数量的棕榈油酸（含量为 16%），可能适用于医疗应用。
