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不同种子油体蛋白稳定的人工油体的物理化学性质和功能的比较分析

Comparative analysis of physicochemical properties and functionalities of artificial oil bodies stabilized by different seed oil body proteins.

作者信息

Yuan Ruhuan, Yi Meixia, Xue Feng, Xiong Xiaohui, Adhikari Benu, Li Chen

机构信息

College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, PR China.

Jiangsu Key Laboratory of Medicinal Substance and Utilization of Fresh Chinese Medicine, School of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, PR China.

出版信息

Int J Biol Macromol. 2025 May;307(Pt 1):141893. doi: 10.1016/j.ijbiomac.2025.141893. Epub 2025 Mar 8.

Abstract

In this study, ‌perilla seed oil body protein (PO), hempseed oil body protein (HO), and cucumber seed oil body protein (CO) were utilized to prepare artificial oil bodies (AOBs), and the impact of protein type on their physicochemical properties was investigated.‌ ‌Compared to HO and CO, PO exhibited a lower zeta potential value of -48.27 mV, a higher surface hydrophobicity of 21,122.67, and a smaller particle size of 105.71 nm. Its emulsifying activity index (EAI) and emulsion stability index (ESI) were 83.06 m/g and 167.9 %, respectively. ‌Furthermore, AOBs stabilized by PO demonstrated smaller particle size and a higher amount of interfacial adsorbed protein compared to those prepared by HO and CO.‌ ‌Additionally, the encapsulation results indicated that PO-AOBs exhibited a higher encapsulation efficiency of 98.34 %. The retention rates of the loaded curcumin were 56.94 % after exposure to 90 °C and 94.06 % after UV light exposure for 7 h. ‌Moreover, both digestion and antioxidant experiments indicated that PO-AOBs facilitated better release of curcumin in the intestine, leading to enhanced antioxidant properties and increased bioavailability.‌ These findings offer ‌new insights into the development of novel AOBs, which hold promise for diverse applications in food delivery systems.

摘要

在本研究中,利用紫苏籽油体蛋白(PO)、大麻籽油体蛋白(HO)和黄瓜籽油体蛋白(CO)制备人工油体(AOBs),并研究了蛋白质类型对其理化性质的影响。与HO和CO相比,PO的ζ电位值较低,为-48.27 mV,表面疏水性较高,为21,122.67,粒径较小,为105.71 nm。其乳化活性指数(EAI)和乳液稳定性指数(ESI)分别为83.06 m/g和167.9%。此外,与由HO和CO制备的AOBs相比,由PO稳定的AOBs粒径更小,界面吸附蛋白量更高。此外,包封结果表明,PO-AOBs表现出更高的包封效率,为98.34%。负载姜黄素在90°C下暴露后的保留率为56.94%,在紫外光下暴露7小时后的保留率为94.06%。此外,消化和抗氧化实验均表明,PO-AOBs有助于姜黄素在肠道中更好地释放,从而增强抗氧化性能并提高生物利用度。这些发现为新型AOBs的开发提供了新的见解,有望在食品递送系统中得到广泛应用。

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