Comby Pierre-Olivier, Guillen Kévin, Chevallier Olivier, Couloumy Emilie, Dencausse Anne, Robert Philippe, Catoen Sarah, Salsac Anne-Virginie, Aho-Glele Serge Ludwig, Loffroy Romaric
Department of Neuroradiology and Emergency Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, 21079, Dijon, France.
ICMUB Laboratory, UMR CNRS 6302, Université de Bourgogne, 210000, Dijon, France.
Cardiovasc Intervent Radiol. 2025 Jun;48(6):823-833. doi: 10.1007/s00270-025-03997-w. Epub 2025 Mar 10.
To compare the radiological and histological effects of three different cyanoacrylate glues used for in vivo renal artery embolization in rabbits.
N-butyl cyanoacrylate (NBCA), NBCA-methacryloxysulfolane (NBCA-MS), and α-hexyl-cyanoacrylate (AHCA) were each used to embolize five renal arteries under free-flow conditions with a 1:3 glue/ethiodized oil ratio. The rabbits were euthanized 30 min after embolization. Micro-computed tomography (CT) was used to assess glue cast distribution (extent of penetration into the vascular tree), fragmentation (presence of discontinuities within the cast), and heterogeneity (variability in cast density). Histological slides were analyzed for lumen dilatation, intimal arteritis, necrosis, and peri-arterial edema. Statistical analyses were performed using the Kruskal-Wallis test.
Glue penetration into interlobar and corticomedullary arteries was observed consistently in all three groups. Fragmentation scores showed no significant differences between glue types, but AHCA demonstrated significantly lower heterogeneity in proximal arteries compared to NBCA-MS (P = 0.019). Severe lumen dilatation and intimal necrosis were observed in all samples, whereas intimal arteritis was significantly less severe with AHCA compared to NBCA-MS (P < 0.001). No significant differences in peri-arterial edema were found between groups.
Micro-CT features were similar with all three glues. AHCA produced greater cast uniformity and less intimal arteritis, suggesting a role in endovascular embolization.
比较三种不同的氰基丙烯酸酯胶水用于兔体内肾动脉栓塞的放射学和组织学效果。
正丁基氰基丙烯酸酯（NBCA）、NBCA-甲基丙烯酰氧基环丁砜（NBCA-MS）和α-己基氰基丙烯酸酯（AHCA）分别在自由流动条件下以胶水/碘化油1:3的比例栓塞五条肾动脉。栓塞后30分钟对兔子实施安乐死。使用微型计算机断层扫描（CT）评估胶水铸型分布（进入血管树的渗透程度）、破碎情况（铸型内有无间断）和异质性（铸型密度的变化）。对组织学切片进行管腔扩张、内膜动脉炎、坏死和动脉周围水肿分析。采用Kruskal-Wallis检验进行统计学分析。
在所有三组中均持续观察到胶水渗入叶间动脉和皮质髓质动脉。破碎评分在不同胶水类型之间无显著差异，但与NBCA-MS相比，AHCA在近端动脉中的异质性显著更低（P = 0.019）。在所有样本中均观察到严重的管腔扩张和内膜坏死，而与NBCA-MS相比，AHCA的内膜动脉炎明显较轻（P < 0.001）。各组之间在动脉周围水肿方面未发现显著差异。
三种胶水的微型CT特征相似。AHCA产生的铸型均匀性更高，内膜动脉炎更少，表明其在血管内栓塞中具有一定作用。
