Ritter Eleanore J, Cochetel Noé, Minio Andrea, Cousins Peter, Cantu Dario, Niederhuth Chad
Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA.
Department of Viticulture and Enology, University of California Davis, Davis, CA 95616, USA.
GigaByte. 2025 Feb 27;2025:gigabyte149. doi: 10.46471/gigabyte.149. eCollection 2025.
Teinturier grapevines, known for their pigmented flesh berries due to anthocyanin production, are valuable for enhancing the pigmentation of wine, for potential health benefits, and for investigating anthocyanin production in plants. Here, we assembled and annotated the Dakapo and Rubired genomes, two teinturier varieties. For Dakapo, we combined Nanopore sequencing, Illumina sequencing, and scaffolding to the existing grapevine assembly to generate a final assembly of 508.5 Mbp. Combining annotation and lifting over annotations from the existing grapevine reference produced annotation 36,940 gene annotations for Dakapo. For Rubired, PacBio HiFi reads were assembled, scaffolded, and phased to generate a diploid assembly with two haplotypes 474.7-476.0 Mbp long. annotation of the diploid Rubired genome yielded annotations for 56,681 genes. Both genomes are highly contiguous and complete. The Dakapo and Rubired genome assemblies provide genetic resources for investigations into berry flesh pigmentation and other traits of interest in grapevine.
因能产生花青素而具有带色果肉浆果的泰恩图里埃葡萄品种，对于增强葡萄酒的色素沉着、潜在的健康益处以及研究植物中的花青素生成具有重要价值。在此，我们组装并注释了两个泰恩图里埃品种——达卡波和鲁比雷的基因组。对于达卡波，我们将纳米孔测序、Illumina测序以及支架构建与现有的葡萄基因组组装相结合，生成了一个508.5兆碱基对的最终组装结果。结合注释以及从现有的葡萄参考基因组中转移过来的注释，为达卡波生成了36,940个基因注释。对于鲁比雷，我们对PacBio HiFi reads进行了组装、构建支架并进行定相，以生成一个具有两个单倍型、长度为474.7 - 476.0兆碱基对的二倍体组装结果。对二倍体鲁比雷基因组的注释产生了56,681个基因的注释。这两个基因组都具有高度的连续性和完整性。达卡波和鲁比雷的基因组组装为研究葡萄浆果果肉色素沉着及其他感兴趣的性状提供了遗传资源。
