Zakut-Houri R, Bienz-Tadmor B, Givol D, Oren M
EMBO J. 1985 May;4(5):1251-5. doi: 10.1002/j.1460-2075.1985.tb03768.x.
A 2.5-kb cDNA clone for human p53 tumor antigen has been isolated. This clone contains the entire coding region including 135 bp upstream of the first ATG. Comparison of the nucleotide sequence of human p53 and mouse p53 demonstrates that the first ATG in human p53 corresponds to the second ATG (codon No. 4) in mouse p53. The human p53 comprises 393 residues and is longer than the mouse p53 due to six additional codons present at the region corresponding to exon 4 of the mouse p53 gene. The DNA sequence homology between the coding regions of mouse and human p53 is 81% and the conservation of homology is not equally distributed along the molecule. When inserted into SV40-based expression vectors the human p53 cDNA successfully directs the production of a polypeptide with an apparent mol. wt. of 55 kd which can be precipitated by monoclonal antibodies to p53.
已分离出人类p53肿瘤抗原的一个2.5kb cDNA克隆。该克隆包含整个编码区，包括第一个ATG上游135bp。人类p53和小鼠p53核苷酸序列的比较表明，人类p53中的第一个ATG对应于小鼠p53中的第二个ATG（第4密码子）。人类p53由393个残基组成，由于在与小鼠p53基因外显子4相对应的区域存在六个额外密码子，因此比小鼠p53长。小鼠和人类p53编码区之间的DNA序列同源性为81%，并且同源性的保守性在分子上分布不均。当插入基于SV40的表达载体时，人类p53 cDNA成功指导产生一种表观分子量为55kd的多肽，该多肽可被p53单克隆抗体沉淀。
