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编码转化相关蛋白p53的全长小鼠cDNA序列的克隆及表达分析

Cloning and expression analysis of full length mouse cDNA sequences encoding the transformation associated protein p53.

作者信息

Jenkins J R, Rudge K, Redmond S, Wade-Evans A

出版信息

Nucleic Acids Res. 1984 Jul 25;12(14):5609-26. doi: 10.1093/nar/12.14.5609.

DOI:10.1093/nar/12.14.5609
PMID:6379601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320018/
Abstract

We have cloned and sequenced overlapping cDNA fragments which together encode the entire mouse protein p53. Using these cDNA's we have reconstructed the full length coding region for the protein, and have analysed its coding potential by expression in vitro, both as a full length sequence and as a subfragment contained in a fusion protein. The predicted amino acid sequence contains no obvious homologies to any known oncogenes but includes a possible tyrosine kinase acceptor site.

摘要

我们已经克隆并测序了重叠的cDNA片段，这些片段共同编码整个小鼠p53蛋白。利用这些cDNA，我们重建了该蛋白的全长编码区，并通过体外表达对其编码潜能进行了分析，包括全长序列以及融合蛋白中包含的一个亚片段。预测的氨基酸序列与任何已知的癌基因均无明显同源性，但包含一个可能的酪氨酸激酶受体位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d590/320018/f0c268d3af30/nar00332-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d590/320018/a4d04aeeefec/nar00332-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d590/320018/7dde619b986c/nar00332-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d590/320018/f0c268d3af30/nar00332-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d590/320018/a4d04aeeefec/nar00332-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d590/320018/7dde619b986c/nar00332-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d590/320018/f0c268d3af30/nar00332-0082-a.jpg

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Cloning and expression analysis of full length mouse cDNA sequences encoding the transformation associated protein p53.编码转化相关蛋白p53的全长小鼠cDNA序列的克隆及表达分析
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J Mol Cell Biol. 2019 Jul 19;11(7):539-543. doi: 10.1093/jmcb/mjz070.
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Posttranslational modification of p53: cooperative integrators of function.p53 的翻译后修饰：功能的协作整合子。

本文引用的文献

1
Lymphocyte stimulation: concanavalin A induces the expression of a 53K protein.淋巴细胞刺激：伴刀豆球蛋白A诱导一种53K蛋白的表达。
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Plasmid screening at high colony density.高菌落密度下的质粒筛选
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A 53-kilodalton protein common to chemically and virally transformed cells shows extensive sequence similarities between species.一种在化学转化和病毒转化细胞中常见的53千道尔顿蛋白质在不同物种间显示出广泛的序列相似性。
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Involvement of transcription factor encoded by the mouse mi locus (MITF) in apoptosis of cultured mast cells induced by removal of interleukin-3.小鼠mi位点（MITF）编码的转录因子参与白细胞介素-3去除诱导的培养肥大细胞凋亡。
Am J Pathol. 1997 Oct;151(4):1043-51.
7
The role of anti-p53-autoantibodies in pancreatic disorders.抗p53自身抗体在胰腺疾病中的作用。
Int J Pancreatol. 1996 Jun;19(3):171-8. doi: 10.1007/BF02787365.
8
Intermolecular mRNA-rRNA hybridization and the distribution of potential interaction regions in murine 18S rRNA.分子间mRNA-rRNA杂交及小鼠18S rRNA中潜在相互作用区域的分布
Nucleic Acids Res. 1993 Feb 25;21(4):1007-11. doi: 10.1093/nar/21.4.1007.
9
Functional characterization of Xenopus laevis p53: evidence of temperature-sensitive transactivation but not of repression.非洲爪蟾p53的功能特性：温度敏感型反式激活而非抑制的证据。
J Virol. 1994 Nov;68(11):7178-87. doi: 10.1128/JVI.68.11.7178-7187.1994.
10
Simian virus 40 large T antigen contains two independent activities that cooperate with a ras oncogene to transform rat embryo fibroblasts.猴病毒40大T抗原含有两种独立的活性，它们与一种ras癌基因协同作用以转化大鼠胚胎成纤维细胞。
J Virol. 1995 Feb;69(2):923-34. doi: 10.1128/JVI.69.2.923-934.1995.
Proc Natl Acad Sci U S A. 1982 Jan;79(2):287-91. doi: 10.1073/pnas.79.2.287.
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A single gene and a pseudogene for the cellular tumour antigen p53.细胞肿瘤抗原p53的一个单基因和一个假基因。
Nature. 1983;306(5943):594-7. doi: 10.1038/306594a0.
5
Role of the p53 protein in cell proliferation as studied by microinjection of monoclonal antibodies.通过显微注射单克隆抗体研究p53蛋白在细胞增殖中的作用。
Mol Cell Biol. 1984 Feb;4(2):276-81. doi: 10.1128/mcb.4.2.276-281.1984.
6
Antibodies of predetermined specificity for the NH2 terminus of a cellular protein p53 react with the native molecule: evidence for the presence of different p53s.对细胞蛋白p53氨基末端具有预定特异性的抗体与天然分子发生反应：存在不同p53的证据。
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1199-203. doi: 10.1073/pnas.80.5.1199.
7
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Gene. 1982 Oct;19(3):269-76. doi: 10.1016/0378-1119(82)90016-6.
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Microinjection of monoclonal antibody to protein p53 inhibits serum-induced DNA synthesis in 3T3 cells.向3T3细胞显微注射针对蛋白p53的单克隆抗体可抑制血清诱导的DNA合成。
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9
Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.酪氨酸磷酸化位点周围氨基酸序列的分析
Proc Natl Acad Sci U S A. 1982 Feb;79(4):973-7. doi: 10.1073/pnas.79.4.973.
10
Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells.腺病毒E1b - 58kd肿瘤抗原和SV40大肿瘤抗原在转化细胞中与同一种54kd细胞蛋白存在物理关联。
Cell. 1982 Feb;28(2):387-94. doi: 10.1016/0092-8674(82)90356-7.