光诱导大鼠肝脏60S核糖体亚基内蛋白质与5S RNA和28 - 5.8S RNA交联

Photo-induced protein cross-linking to 5S RNA and 28-5.8S RNA within rat-liver 60S ribosomal subunits.

作者信息

Paleologue A, Rebound A M, Rebound J P

出版信息

Eur J Biochem. 1985 Jun 18;149(3):525-9. doi: 10.1111/j.1432-1033.1985.tb08956.x.

DOI:10.1111/j.1432-1033.1985.tb08956.x

Abstract

Rat liver 60S ribosomal subunits were irradiated with 254-nm ultraviolet light (1.26 X 10(4) quanta/subunit), under conditions which preserved their functional activity. Cross-linked RNA-protein complexes were recovered after unreacted proteins had been removed by repeated acetic acid extractions. Proteins linked to the whole rRNA, to 5S RNA and to 28-5.8 S RNAs were identified by two-dimensional gel electrophoresis after RNA hydrolysis by ribonucleases T1 and A. Our results showed that numerous proteins interact with rRNAs (at least ten with 28-5.8 S RNA, eight with 5S RNA and among these three are common to both) and have been discussed in the light of all the available data.

摘要

在保留其功能活性的条件下，用254纳米紫外线（1.26×10⁴量子/亚基）照射大鼠肝脏60S核糖体亚基。在用乙酸反复萃取除去未反应的蛋白质后，回收交联的RNA-蛋白质复合物。在核糖核酸酶T1和A水解RNA后，通过二维凝胶电泳鉴定与整个rRNA、5S RNA和28-5.8 S RNA相连的蛋白质。我们的结果表明，许多蛋白质与rRNA相互作用（至少十种与28-5.8 S RNA相互作用，八种与5S RNA相互作用，其中三种是两者共有的），并已根据所有现有数据进行了讨论。