翻译系统中的多核苷酸 - 蛋白质相互作用。大肠杆菌核糖体30-S亚基中一些核糖体分裂蛋白与16-S RNA之间接触的检测。

Polynucleotide-protein interactions in the translation system. Detection of contacts between some ribosomal split proteins and 16-S RNA in 30-S subunits of Escherichia coli ribosomes.

作者信息

Turchinsky M F, Broude N E, Kussova K S, Abduraschidova G G, Muchamedganova E V, Schatsky I N, Bystrova T F, Budowsky E I

出版信息

Eur J Biochem. 1978 Sep 15;90(1):83-8. doi: 10.1111/j.1432-1033.1978.tb12577.x.

DOI:10.1111/j.1432-1033.1978.tb12577.x

PMID:361396

Abstract

Direct contacts between 16-S RNA and split proteins S2, S3, S5, S14 and S21 inside the 30-S subunit of Escherichia coli ribosomes were evidenced by the formation of ultraviolet-induced (lambda = 254 nm) RNA-protein cross-links. 30-S subunits were reassembled from core particles and a mixture of split proteins containing in each case a single 125I-labelled protein. All the proteins tested are cross-linked as a result of a single-hit process; proteins S3 and S21 were cross-linked at the highest rate.

摘要

通过紫外线诱导（波长=254nm）的RNA-蛋白质交联反应，证实了大肠杆菌核糖体30-S亚基内部16-S RNA与裂解蛋白S2、S3、S5、S14和S21之间存在直接接触。30-S亚基由核心颗粒和每种情况下都含有一种125I标记蛋白的裂解蛋白混合物重新组装而成。所有测试的蛋白都通过单击中过程发生交联；蛋白S3和S21的交联速率最高。