Francis D, Davies N D, Bryant J A, Hughes S G, Sibson D R, Fitchett P N
Exp Cell Res. 1985 Jun;158(2):500-8. doi: 10.1016/0014-4827(85)90473-2.
We have examined by fibre autoradiography the spacing of replicons in pea root meristems during synchronized entry into S phase from arrest at the G1/S boundary. Pretreatment with the DNA cross-linking agent, psoralen, produces a marked shortening of replicon spacing, suggesting that premature arrest of the replication fork results in the recruitment of additional initiation points within a given replicon family. This is discussed in relation to models for the control of DNA replication.
我们通过纤维放射自显影术研究了豌豆根分生组织在从G1/S边界停滞同步进入S期过程中复制子的间距。用DNA交联剂补骨脂素进行预处理会使复制子间距显著缩短，这表明复制叉的过早停滞会导致在给定的复制子家族中招募额外的起始点。本文结合DNA复制控制模型对此进行了讨论。
