Razin S V, Kekelidze M G, Lukanidin E M, Scherrer K, Georgiev G P
Nucleic Acids Res. 1986 Oct 24;14(20):8189-207. doi: 10.1093/nar/14.20.8189.
DNA fragments containing replication origins (oriDNA) were isolated from a chicken erythroblast cell line by a modified procedure of Zannis-Hadjopoulos et al. and studied in the renaturation reaction driven by either total or nuclear matrix DNA (nmDNA) from the same cells or from mature erythrocytes. We found that the unique sequences of nmDNA from erythroblasts (5 kb long) represented a specific subset of sequences constituting about a quarter of total DNA unique sequences, while the erythrocyte nmDNA 5 kb fragments constitute only about one tenth of total unique DNA and all are recovered among erythroblast nmDNA. Virtually all oriDNA sequences are present in the fraction of erythrocyte nmDNA. Thereafter, the putative positions of replication origins within the alpha-globine gene domain have been mapped by hybridization experiments. They were found to coincide with the previously established positions of permanent sites of DNA attachment to the nuclear matrix.
通过对赞尼斯 - 哈乔普洛斯等人的方法进行改进，从鸡成红细胞系中分离出含有复制起点的DNA片段（oriDNA），并在由同一细胞或成熟红细胞的总DNA或核基质DNA（nmDNA）驱动的复性反应中进行研究。我们发现，成红细胞nmDNA的独特序列（5kb长）代表了构成总DNA独特序列约四分之一的特定序列子集，而成红细胞nmDNA的5kb片段仅占总独特DNA的约十分之一，并且所有这些片段都能在成红细胞nmDNA中找到。实际上，所有oriDNA序列都存在于红细胞nmDNA部分中。此后，通过杂交实验绘制了α - 珠蛋白基因域内复制起点的假定位置。发现它们与先前确定的DNA附着于核基质的永久位点位置一致。
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