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采用平衡检测的可见光偏振敏感光学相干断层扫描技术

Visible light polarization-sensitive optical coherence tomography with balanced detection.

作者信息

Baradaran Bahar, Black Adam J, Heilbronner Sarah R, Akkin Taner

机构信息

University of Minnesota, Department of Biomedical Engineering, Minneapolis, Minnesota, United States.

Baylor College of Medicine, Department of Neurosurgery, Houston, Texas, United States.

出版信息

J Biomed Opt. 2025 Mar;30(3):036002. doi: 10.1117/1.JBO.30.3.036002. Epub 2025 Mar 11.

Abstract

SIGNIFICANCE

We introduce a visible-light polarization-sensitive optical coherence tomography (PS-OCT) system that operates in the spectral domain with balanced detection (BD) capability. While the BD improves the signal-to-noise ratio (SNR), the use of shorter wavelengths improves spatial resolution and birefringence sensitivity.

AIM

We aim to implement a new optical design, characterize its performance, and investigate the imaging potential for biological tissues.

APPROACH

The design utilizes a unique interferometer and a custom spectrometer that captures four highly aligned spectra with a single area/multi-line camera. Each pair of spectral lines is highly aligned, and their subtraction yields balanced detected spectra of the PS-OCT channels. The resulting channels provide multiple imaging contrasts.

RESULTS

We measured the axial resolution and quantified the BD performance within the imaging depth. We also used a variable retarder to characterize the phase retardance and optic axis orientation measurements. Imaging results demonstrate the expected improvements for biological tissue.

CONCLUSIONS

We successfully implemented BD for a high-resolution visible-light PS-OCT. Improved SNR and birefringence sensitivity allow better delineation of birefringent structures in biological tissues. This opens up new opportunities in the biomedical imaging field, especially for resolving structures and fibers that exhibit birefringence.

摘要

意义

我们介绍了一种在光谱域中运行且具有平衡检测(BD)能力的可见光偏振敏感光学相干断层扫描(PS-OCT)系统。虽然平衡检测提高了信噪比(SNR),但使用较短波长可提高空间分辨率和双折射灵敏度。

目的

我们旨在实现一种新的光学设计,表征其性能,并研究其对生物组织的成像潜力。

方法

该设计采用了一种独特的干涉仪和定制光谱仪,通过单区域/多线相机捕获四条高度对齐的光谱。每对光谱线高度对齐,它们相减可得到PS-OCT通道的平衡检测光谱。所得通道提供多种成像对比度。

结果

我们测量了轴向分辨率,并在成像深度范围内量化了平衡检测性能。我们还使用可变延迟器来表征相位延迟和光轴方向测量。成像结果证明了对生物组织预期的改善效果。

结论

我们成功地为高分辨率可见光PS-OCT实现了平衡检测。改善的信噪比和双折射灵敏度能够更好地描绘生物组织中的双折射结构。这为生物医学成像领域开辟了新机会,特别是在解析呈现双折射的结构和纤维方面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/321e/11896081/5bc9061dfee2/JBO-030-036002-g001.jpg

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