Goudou D, Verdiere-Sahuque M, Rieger F
FEBS Lett. 1985 Jul 1;186(1):54-8. doi: 10.1016/0014-5793(85)81338-7.
The subcellular distribution of multiple molecular forms of acetylcholinesterase (AChE) in neurones of rat superior cervical ganglion (SCG) was determined both in vivo and in vitro by the use of selective lipid-soluble or -insoluble inhibitors. In vivo as well as in vitro, 10 S AChE is mainly outside the cell. In primary cultures of rat SCG neurones, both 4 S and 16 S AChE are mainly inside the cell. In near-term rat SCG, 4 S and 16 S are more external to the cell than in primary cultures. In adult rat SCG, 4 S AChE is equally distributed inside and outside and 16 S AChE is mainly outside the cell. Thus, specific AChE externalization probably occurs in neuronal cells as a developmentally regulated process.
利用选择性脂溶性或非脂溶性抑制剂，在体内和体外测定了大鼠颈上神经节（SCG）神经元中多种分子形式的乙酰胆碱酯酶（AChE）的亚细胞分布。在体内和体外，10S AChE主要位于细胞外。在大鼠SCG神经元的原代培养物中，4S和16S AChE主要位于细胞内。在接近足月的大鼠SCG中，4S和16S比在原代培养物中更位于细胞外。在成年大鼠SCG中，4S AChE在细胞内外均匀分布，16S AChE主要位于细胞外。因此，特定的AChE外化可能作为一种发育调节过程发生在神经元细胞中。
