MacDonald C M, Freshney R I, Hart E, Graham D I
Exp Cell Biol. 1985;53(3):130-7. doi: 10.1159/000163305.
Cells cultured from anaplastic astrocytoma (Kernohan and Sayre, grades III and IV) will proliferate on confluent monolayers of normal glia, while cells cultured from normal brain will not. The growth of a cell line containing a high proportion of well-differentiated glioma cells (G-CCM) was partially inhibited, though not as much as normal glia, while the growth of a cell line made up of less differentiated cells (G-UVW) was enhanced by the normal glia. Although non-glial confluent monolayers also inhibited the growth of normal glia, this was less specific, as one normal glial line (N-DUT) grew on fibroblasts and intestinal epithelium, although it was unable to do so on normal glia. It is suggested that this may be a useful method for examining reduced density limitation of growth, discriminating between normal and malignant glia, and for separating glioma cells from contaminating normal cells.
间变性星形细胞瘤（Kernohan和Sayre分级为III级和IV级）培养的细胞会在正常神经胶质细胞的汇合单层上增殖，而正常脑组织培养的细胞则不会。含有高比例分化良好的胶质瘤细胞的细胞系（G-CCM）的生长受到部分抑制，尽管不如正常神经胶质细胞那么明显，而由分化程度较低的细胞组成的细胞系（G-UVW）的生长则因正常神经胶质细胞而增强。虽然非神经胶质细胞的汇合单层也会抑制正常神经胶质细胞的生长，但这种抑制作用不太具有特异性，因为一种正常神经胶质细胞系（N-DUT）能在成纤维细胞和肠上皮细胞上生长，尽管它在正常神经胶质细胞上无法生长。有人认为，这可能是一种用于检查生长密度限制降低、区分正常和恶性神经胶质细胞以及从污染的正常细胞中分离胶质瘤细胞的有用方法。
