Collins V P, Forsby N, Brunk U T, Ericsson J L, Westermark B
Acta Pathol Microbiol Scand A. 1979 Jan;87(1):19-28. doi: 10.1111/j.1699-0463.1979.tb00019.x.
The fine structure of three lines of human normal glial cells and eight established lines of malignant glioma cells are described. The glial cell lines were ultrastructurally very similar whereas the glioma cell lines differed greatly from one another. In sparse proliferating cultures there were no consistent findings which distinguished the glioma cell lines as a group from the normal glial cells. Only in post-confluent cultures could the consistently irregular cell surfaces and ruffling, both at the cell periphery and centrally on the upper cell surface, with associated pinocytosis, distinguish the glioma from the post-confluent glial cultures, which did not possess these properties. The common attributes of post-confluent glioma cells reflect the cells' continued proliferation. The glioma lines did display individual ultrastructural characteristics which appear to be stable, the glioma lines having retained these during a number of years of continual passage.
本文描述了三株人正常神经胶质细胞系和八株已建立的恶性神经胶质瘤细胞系的精细结构。神经胶质细胞系在超微结构上非常相似，而神经胶质瘤细胞系彼此之间差异很大。在稀疏增殖培养中，没有一致的发现能将神经胶质瘤细胞系作为一个群体与正常神经胶质细胞区分开来。只有在汇合后培养中，细胞周边和上表面中央持续出现的不规则细胞表面和褶皱，以及相关的胞饮作用，才能将神经胶质瘤与不具备这些特性的汇合后神经胶质细胞培养区分开来。汇合后神经胶质瘤细胞的共同特征反映了细胞的持续增殖。神经胶质瘤细胞系确实表现出似乎稳定的个体超微结构特征，这些神经胶质瘤细胞系在多年的连续传代过程中一直保留着这些特征。
