Colbére-Garapin F, Horaud F, Kourilsky P, Garapin A C
Dev Biol Stand. 1985;59:109-12.
After transfection of Vero cells with the hepatitis B surface (HBs) antigen gene and the dominant selective marker amino-glycoside 3' phosphotransferase (APH3'), a late transient HBs expression is observed during the first weeks after transfection. We isolated 300 G418-resistant cell clones transformed by plasmids carrying the two genes. Only 6% of them were found to express the HBs antigen stably (versus 65% in murine cells). The HBs antigen expression is maximal after cells have reached confluency, and this level of antigen expression remains stable for months when cells are fed twice weekly without trypsinization. One clone - GAR 1412 - has been studied in greater detail. The cells (10(6] are not tumorigenic for nude mice, and the excreted HBs antigen, which is under the form 22 nm spherical particles, is immunogenic in Balb/C mice.
用乙型肝炎表面（HBs）抗原基因和显性选择标记氨基糖苷3'磷酸转移酶（APH3'）转染Vero细胞后，在转染后的最初几周观察到短暂的晚期HBs表达。我们分离出了300个由携带这两个基因的质粒转化的G418抗性细胞克隆。其中只有6%被发现能稳定表达HBs抗原（而在鼠细胞中这一比例为65%）。细胞达到汇合状态后，HBs抗原表达达到最大值，当细胞每周喂养两次且不进行胰蛋白酶消化时，这种抗原表达水平可稳定数月。对一个克隆——GAR 1412——进行了更详细的研究。这些细胞（10⁶）对裸鼠无致瘤性，分泌的呈22纳米球形颗粒形式的HBs抗原在Balb/C小鼠中具有免疫原性。
