Colbère-Garapin F, Horaud F, Kourilsky P, Garapin A
J Gen Virol. 1985 Aug;66 ( Pt 8):1741-52. doi: 10.1099/0022-1317-66-8-1741.
In this paper, we show that the pattern of expression of the human hepatitis B surface antigen (HBs Ag) gene, transfected along with a dominant selectable marker into mammalian cells, is complex. In human hepatoma (HepG2) cells, late transient expression occurs and permanent expression takes place at high frequencies in the selected clones. In HeLa and human xeroderma pigmentosum (GM4312A)-derived cells, the late transient expression is barely seen or absent and permanent expression is only seen in a few selected clones. In monkey kidney Vero cells, late transient expression has been described and we show in this report that only 5% of the selected clones are capable of expressing HBs Ag in a permanent manner. In most of the Vero clones, the absence of HBs Ag expression is mainly due to HBs Ag gene rearrangements. We have selected and amplified more than 500 transfected Vero clones and have characterized in detail one clone (GAR1412) which is a permanent high-level HBs Ag expressor.
在本文中，我们表明，与显性选择标记一起转染到哺乳动物细胞中的人乙型肝炎表面抗原（HBs Ag）基因的表达模式是复杂的。在人肝癌（HepG2）细胞中，会出现晚期瞬时表达，并且在选定的克隆中高频发生永久表达。在源自HeLa和人着色性干皮病（GM4312A）的细胞中，几乎看不到或不存在晚期瞬时表达，并且仅在少数选定的克隆中观察到永久表达。在猴肾Vero细胞中，已描述了晚期瞬时表达，并且我们在本报告中表明，仅5%的选定克隆能够永久表达HBs Ag。在大多数Vero克隆中，HBs Ag表达缺失主要是由于HBs Ag基因重排。我们已筛选并扩增了500多个转染的Vero克隆，并详细鉴定了一个克隆（GAR1412），它是一个永久的高水平HBs Ag表达者。
