Goso K, Ishihara K, Ohara S, Kakei M, Hotta K
Digestion. 1985;31(4):205-12. doi: 10.1159/000199201.
Radiolabeled glycoconjugates were prepared by incorporation of [35S]sulfate and [3H]glucosamine into the gastric mucosa of human gastric resection specimens, using organ culture techniques. Specimens which incorporated higher amounts of [35S]sulfate into high molecular glycoconjugates (Fr-1) were designated as group I. The average specific activity in Fr-1 of group I was 30 times that of group II, but the glucosamine uptake remained about the same in all cases. All specimens in group I were positive to intestinal metaplasia in their adjacent mucosal area. The sulfated glycoproteins in Fr-1 of group I were found to incorporate 80% of the 35S and the remaining 20% by sulfated glycosaminoglycans. But the glycoconjugates constituting all the Fr-1 of group II were identified as glycoproteins and incorporating 35S only to a small extent were identified as sulfated glycoproteins. The susceptibility of the glycoproteins from group I mucosa to pronase digestion was different from that of group II. The glycoproteins synthesized in group I mucosa were as sulfated glycoproteins as well as nonsulfated glycoproteins and glycosaminoglycans, while the group II glycoproteins consisted primarily of nonsulfated glycoproteins.
采用器官培养技术，通过将[35S]硫酸盐和[3H]葡糖胺掺入人胃切除标本的胃黏膜中来制备放射性标记的糖缀合物。将向高分子量糖缀合物（组分1）中掺入较高量[35S]硫酸盐的标本指定为I组。I组组分1中的平均比活性是II组的30倍，但在所有情况下葡糖胺摄取量保持大致相同。I组的所有标本在其相邻黏膜区域均呈肠化生阳性。发现I组组分1中的硫酸化糖蛋白掺入了80%的35S，其余20%由硫酸化糖胺聚糖掺入。但构成II组所有组分1的糖缀合物被鉴定为糖蛋白，仅少量掺入35S的被鉴定为硫酸化糖蛋白。I组黏膜中的糖蛋白对链霉蛋白酶消化的敏感性与II组不同。I组黏膜中合成的糖蛋白既有硫酸化糖蛋白，也有非硫酸化糖蛋白和糖胺聚糖，而II组糖蛋白主要由非硫酸化糖蛋白组成。
