Heifetz A, Prager M D
J Biol Chem. 1981 Jul 10;256(13):6529-32.
Human kidney tumor cells in culture incorporate [3H]glucosamine and 35SO4 into several classes of glycoconjugate products. After a 24-h labeling period, the 3H/35S-glycosaminoglycans and 3H-glycoproteins synthesized were found associated with both the cell layer and the culture medium. These tumor cells also synthesized a class of 35S-glycoproteins which contained alkali-stable 35S-oligosaccharide chains. These 35S-glycoproteins did not accumulate with the cell layers but were preferentially found in the culture medium (82-96%). After treatment with 2.5 mM butyrate for 24 h, the tumor cells assumed a more flattened and spread morphology with more clearly defined cell borders. These butyrate-treated cell cultures showed less than a 2-fold increase in the cell-associated form of both 3H/35S-glycosaminoglycans and 3H-glycoproteins, compared to cells cultured in the absence of butyrate. In contrast, butyrate-treated cell cultures had a 3-fold increase in the total incorporation of 35SO4 into glycoproteins and a dramatic 10-30-fold increase in the cell-associated form of these 35S-glycoprotein products as compared to untreated cell cultures.
培养的人肾肿瘤细胞将[3H]葡糖胺和35SO4掺入几类糖缀合物产物中。经过24小时的标记期后，发现合成的3H/35S-糖胺聚糖和3H-糖蛋白与细胞层和培养基都有关联。这些肿瘤细胞还合成了一类含有碱稳定的35S-寡糖链的35S-糖蛋白。这些35S-糖蛋白没有在细胞层中积累，而是优先存在于培养基中（82-96%）。用2.5 mM丁酸盐处理24小时后，肿瘤细胞呈现出更扁平且伸展的形态，细胞边界更清晰。与在无丁酸盐条件下培养的细胞相比，这些经丁酸盐处理的细胞培养物中，3H/35S-糖胺聚糖和3H-糖蛋白与细胞相关形式的增加不到2倍。相反，与未处理的细胞培养物相比，经丁酸盐处理的细胞培养物中35SO4掺入糖蛋白的总量增加了3倍，这些35S-糖蛋白产物与细胞相关形式显著增加了10-30倍。
