Xu Jiaqi, Li Wenrui, Sun Min, Li Qianyu, Jiang Fan, Wu Yongning, Li Guoliang
School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
NHC Key Laboratory of Food Safety Risk Assessment, Food Safety Research Unit (2019RU014) of Chinese Academy of Medical Science, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
J Chromatogr A. 2025 May 10;1748:465827. doi: 10.1016/j.chroma.2025.465827. Epub 2025 Feb 28.
Endocrine disrupting chemicals (EDCs) have received significant attention in the food field due to their potential health risks. Herein, we proposed a novel core-shell structure magnetic cationic covalent organic framework (EB-DHTA-iCOF@FeO) designed for the efficient enrichment of trace-level EDCs in foodstuffs and analyzed using HPLC-MS/MS. Due to the phenolic EDCs structure possessing hydroxyl functional groups which become protonated under alkaline conditions, resulting in the formation of negatively charged anions. The EB-DHTA-iCOF@FeO positively charged surface can have a good enrichment effect on EDCs with phenolic structures through electrostatic interactions, π - π interactions, and hydrogen bonding. This unique combination of interactions enhances the iCOF ability to selectively capture and enrich phenolic EDCs from complex matrices, thereby improving the sensitivity and efficiency of their detection in analytical applications. Under optimal magnetic solid-phase extraction (MSPE) conditions, the method showed excellent linearity (5-250 μg kg, R ≥ 0.9993) and a low detection limit (0.03-1.2 μg kg) for phenolic EDCs, with recovery rates between 86.0 % and 106.8 % and a relative standard deviation under 5.8 %. The approach highlights the potential of the ionic covalent organic framework as an adsorbent for MSPE, offering a promising approach for the detection and analysis of trace-level EDCs in foodstuffs.
内分泌干扰化学物质（EDCs）因其潜在的健康风险而在食品领域受到了广泛关注。在此，我们提出了一种新型的核壳结构磁性阳离子共价有机框架（EB-DHTA-iCOF@FeO），用于高效富集食品中的痕量EDCs，并采用高效液相色谱-串联质谱（HPLC-MS/MS）进行分析。由于酚类EDCs结构中含有羟基官能团，在碱性条件下会发生质子化，从而形成带负电荷的阴离子。EB-DHTA-iCOF@FeO带正电荷的表面可以通过静电相互作用、π-π相互作用和氢键对具有酚类结构的EDCs产生良好的富集效果。这种独特的相互作用组合增强了iCOF从复杂基质中选择性捕获和富集酚类EDCs的能力，从而提高了其在分析应用中的检测灵敏度和效率。在最佳磁性固相萃取（MSPE）条件下，该方法对酚类EDCs表现出优异的线性（5-250 μg/kg，R≥0.9993）和低检测限（0.03-1.2 μg/kg），回收率在86.0%至106.8%之间，相对标准偏差低于5.8%。该方法突出了离子共价有机框架作为MSPE吸附剂的潜力，为食品中痕量EDCs的检测和分析提供了一种有前景的方法。
