Sue M W, Guth P H
Gastroenterology. 1985 Aug;89(2):415-20. doi: 10.1016/0016-5085(85)90345-2.
A new sensitive in vivo fluorescent method to assess gastric mucosal integrity in the anesthetized rat is described. Topically applied fluorescein diacetate enters gastric mucosal cells. The diacetate is cleaved by intracellular esterases leaving fluorescein trapped within the cells. The pattern of fluorescence can be visualized, and the intensity of fluorescence measured, using a fluorescent in vivo microscopy system. Frozen section studies revealed that fluorescein was located in the surface mucous cells and the mucous neck cells. Topically applied ethanol caused a dose-dependent decline in intensity of fluorescence. Measurement of fluorescence in the supernatant bathing the mucosa revealed that leak of fluorescein out of cells or shedding of cells was, at least in part, responsible for the decline in fluorescence intensity. Pretreatment with a "cytoprotective" dose of 16,16-dimethyl prostaglandin E2 did not protect against the decline in fluorescence seen after 12.5% and 25% ethanol. This confirms findings of previous histologic studies that prostaglandin "cytoprotection" does not include surface cell protection. We conclude that this technique provides a sensitive, quantitative in vivo method to study gastric surface cell injury.
本文描述了一种用于评估麻醉大鼠胃黏膜完整性的新型灵敏体内荧光方法。局部应用的荧光素二乙酸酯进入胃黏膜细胞。二乙酸酯被细胞内酯酶裂解，使荧光素被困在细胞内。使用体内荧光显微镜系统可以观察荧光模式并测量荧光强度。冰冻切片研究表明，荧光素位于表面黏液细胞和黏液颈细胞中。局部应用乙醇导致荧光强度呈剂量依赖性下降。对黏膜周围上清液中的荧光进行测量发现，荧光素从细胞中泄漏或细胞脱落至少部分导致了荧光强度的下降。用“细胞保护”剂量的16,16 - 二甲基前列腺素E2预处理并不能防止在12.5%和25%乙醇处理后出现的荧光下降。这证实了先前组织学研究的结果，即前列腺素的“细胞保护”不包括对表面细胞的保护。我们得出结论，该技术提供了一种灵敏的、定量的体内方法来研究胃表面细胞损伤。
