Liu Mijia, Fu Shijiao, Xu Jiayan, Yuan Chenyi, Ma Jianwei, Gu Chenjie, Jiang Tao, Bian Xueyan
Department of Nephrology, The First Affiliated Hospital of Ningbo University, Ningbo, 315010, Zhejiang, PR China.
School of Physical Science and Technology, Ningbo University, Ningbo, 315211, Zhejiang, PR China.
Anal Chim Acta. 2025 May 1;1349:343829. doi: 10.1016/j.aca.2025.343829. Epub 2025 Feb 22.
The sensitive, efficient, and simultaneous assay of creatinine and urea in different body fluid is crucial for the daily detection and treatment of chronic kidney disease. Here, we exploited a versatile composite surface enhanced Raman scattering (SERS) substrate of polydimethylsiloxane (PDMS)-flower-like ZIF-67@Ag nanoparticles (NPs) based on simple in-situ growth and ion sputtering strategies. The plasmonic Ag NPs assembled on the three-dimensional anisotropic ZIF-67 matrix, facilitating numerous resonant electromagnetic "hotspots". Furthermore, the effective photo-induced charge transfer between target molecules and the flower-like ZIF-67 provide additional chemical enhancement for the assay. Moreover, after a limit of detection as about 8.94 × 10 M was achieved for model ligand of methylene blue, the PDMS-flower-like ZIF-67@AgNPs with an optimal synergistic enhanced SERS activity was able to simultaneously monitor creatinine and urea mixed in artificial clinical samples at different concentration ratios. Significantly, the artificial clinical samples were sequentially detected by using the versatile SERS substrate under different modes of test-strip dipping for blood, hydrophilic-hydrophobic enrichment for urine, and in-situ collection for sweat, which all demonstrated reliable recovery rates. This indicated that SERS technique based on sophisticated nanocomposites has emerged as an alternative protocol to predict kidney disease.
对不同体液中的肌酐和尿素进行灵敏、高效且同步的检测，对于慢性肾病的日常诊断和治疗至关重要。在此，我们基于简单的原位生长和离子溅射策略，开发了一种由聚二甲基硅氧烷（PDMS）-花状ZIF-67@Ag纳米颗粒（NPs）组成的多功能复合表面增强拉曼散射（SERS）基底。等离子体Ag NPs组装在三维各向异性的ZIF-67基质上，形成了大量的共振电磁“热点”。此外，目标分子与花状ZIF-67之间有效的光致电荷转移为检测提供了额外的化学增强作用。此外，在对亚甲基蓝模型配体实现约8.94×10⁻⁶ M的检测限后，具有最佳协同增强SERS活性的PDMS-花状ZIF-67@Ag NPs能够同时监测不同浓度比的人工临床样本中混合的肌酐和尿素。值得注意的是，通过使用这种多功能SERS基底，在不同的测试条浸入血液模式、尿液的亲水-疏水富集模式和汗液的原位收集模式下对人工临床样本进行了顺序检测，所有这些都显示出可靠的回收率。这表明基于精密纳米复合材料的SERS技术已成为预测肾病的一种替代方案。
