School of Physical Science and Technology, Ningbo University, Ningbo, Zhejiang 315211, PR China.
Department of Urology, Ningbo Clinical Research Center for Urological Disease, The First Affiliated Hospital of Ningbo University, Ningbo University, Ningbo, Zhejiang 315010, PR China.
Colloids Surf B Biointerfaces. 2024 Jul;239:113963. doi: 10.1016/j.colsurfb.2024.113963. Epub 2024 May 16.
Among various biomimetic polymer materials, polydimethylsiloxane (PDMS) stands out as an ideal matrix for surface-enhanced Raman scattering (SERS) due to its unique intrinsic Raman signal and tenacity. In order to realize the precise detection of prostate-specific antigen (PSA), we proposed a sandwich-type SERS-active immunostructure composed of PDMS@silver nanoparticles (Ag NPs)@ZIF-67 biomimetic film as the immunosubstrate and gold nanorods (Au NRs) as immunoprobes. Due to the synergistic effect of electromagnetic enhancement facilitated by biomimetic surfaces and chemical enhancement achieved by ZIF-67, this structure enabled an ultrasensitive and selective detection of PSA across a broad range from 10 to 10 mg/mL. The achieved limit of detection was as low as 3.0 × 10 mg/mL. Particularly, the intrinsic Raman signal of PDMS matrix at 2905 cm was employed as a potential internal standard (IS) in the detection, achieving a high coefficient of determination (R) value of 0.996. This multifunctional SERS substrate-mediated immunoassay holds vast potential for early diagnosis of prostate cancer, offering promising prospects for clinical applications.
在各种仿生聚合物材料中，聚二甲基硅氧烷（PDMS）因其独特的固有拉曼信号和韧性而成为表面增强 Raman 散射（SERS）的理想基质。为了实现对前列腺特异性抗原（PSA）的精确检测，我们提出了一种由 PDMS@银纳米粒子（Ag NPs）@ZIF-67 仿生膜作为免疫基底和金纳米棒（Au NRs）作为免疫探针组成的三明治型 SERS 活性免疫结构。由于仿生表面促进的电磁增强和 ZIF-67 实现的化学增强的协同作用，该结构能够在 10 至 10 mg/mL 的较宽范围内对 PSA 进行超灵敏和选择性检测。实现的检测限低至 3.0×10 mg/mL。特别地，在检测中使用 PDMS 基质的固有 Raman 信号（2905 cm）作为潜在的内标（IS），实现了 0.996 的高决定系数（R）值。这种多功能 SERS 基底介导的免疫分析方法为前列腺癌的早期诊断提供了广阔的应用前景，具有很大的临床应用潜力。
