Bourne A R, Taylor J L, Watson T G
Gen Comp Endocrinol. 1985 Jun;58(3):394-401. doi: 10.1016/0016-6480(85)90111-x.
Blood and testicular extracts of the scincid lizard Tiliqua (Trachydosaurus) rugosa were analyzed using thin-layer, column, and high-performance liquid chromatography. The major androgens isolated were epitestosterone, testosterone, and androstenedione. Epitestosterone was characterized by chromatography in several systems, and by derivative formation. Epitestosterone was further identified in testicular extracts by gas chromatographymass spectrometry. Immunoreactive material was detected in tissue extracts using an antiserum specific to epitestosterone. The maximum concentration of epitestosterone in blood, measured by radioimmunoassay, was four times that of testosterone (approximately 900 and 200 nmol/l, respectively). Epitestosterone could not be detected in the blood of intact females and the concentration of this steroid was low in castrate males. The maximum testicular concentrations (pmol/testis) were 390 (nonincubated) and 2050 (incubated) for epitestosterone, and 2025 (nonincubated) and 1040 (incubated) for testosterone. Both plasma and testicular concentrations showed considerable seasonal variation. The identification of endogenous epitestosterone confirms the results of earlier investigations using radioactive substrates. The occurrence of this steroid as a major product of the testis in T. rugosa is discussed in relation to androgens in reptiles and other vertebrates.
利用薄层色谱法、柱色谱法和高效液相色谱法对褶尾蜥（糙鳞沙蜥）的血液和睾丸提取物进行了分析。分离出的主要雄激素为表睾酮、睾酮和雄烯二酮。通过在多个系统中的色谱分析以及衍生物的形成对表睾酮进行了表征。通过气相色谱 - 质谱联用仪在睾丸提取物中进一步鉴定了表睾酮。使用针对表睾酮的抗血清在组织提取物中检测到了免疫反应性物质。通过放射免疫测定法测得血液中表睾酮的最大浓度是睾酮的四倍（分别约为900和200 nmol/l）。在完整雌性动物的血液中未检测到表睾酮，去势雄性动物中该类固醇的浓度较低。表睾酮的最大睾丸浓度（pmol/睾丸）分别为390（未孵育）和2050（孵育），睾酮的最大睾丸浓度分别为2025（未孵育）和1040（孵育）。血浆和睾丸浓度均表现出明显的季节性变化。内源性表睾酮的鉴定证实了早期使用放射性底物进行的研究结果。结合爬行动物和其他脊椎动物中的雄激素，讨论了这种类固醇作为褶尾蜥睾丸主要产物的存在情况。
