[通过垂直聚丙烯酰胺凝胶电泳法测定3型补体成分（C'3）的基因多态性]

[Determination of the genetic polymorphism of the 3d complement component (C'3) by vertical polyacrylamide gel electrophoresis].

作者信息

Batsuur' Zh, Petrishchev V N, Rautian G S, Shneĭder Iu V

出版信息

Genetika. 1985 Apr;21(4):658-63.

Abstract

A variant of the vertical polyacrylamide gel electrophoresis method, hitherto not applied in determination of C'3, is used. The method allows simultaneous determination of the C'3 phenotypes and other serum proteins in human blood. In this paper, the influence of aging of the serum samples and of the Tf polymorphism on the C'3 electrophoregram is discussed. The gene frequencies in Mongolian population (MPR) are: C'3S = 0.9605, C'3F = 0.0375, and the rare variants C'3Srare = 0.0013, C'3Frare = 0.0007; in Chukchi these are 1.0, 0.0, 0.0, respectively; in Eskimos - 0.9556, 0.0222, 0.0111; Evenki - 0.9359, 0.0641, 0.0; Yakuts - 0.9091, 0.0909, 0.0; Komi-Zyrians - 0.8507, 0.1493, 0.0; Komi-Permiyak - 0.8176, 0.1698, 0.0068.

摘要

采用了一种垂直聚丙烯酰胺凝胶电泳方法的变体，该方法迄今尚未应用于C'3的测定。该方法可同时测定人血中的C'3表型和其他血清蛋白。本文讨论了血清样本老化和转铁蛋白多态性对C'3电泳图谱的影响。蒙古族人群（MPR）的基因频率为：C'3S = 0.9605，C'3F = 0.0375，罕见变体C'3Srare = 0.0013，C'3Frare = 0.0007；楚科奇人分别为1.0、0.0、0.0；爱斯基摩人为0.9556、0.0222、0.0111；鄂温克人为0.9359、0.0641、0.0；雅库特人为0.9091、0.0909、0.0；科米-齐良人为0.8507、0.1493、0.0；科米-彼尔米亚克人为0.8176、0.1698、0.0068。