Peng Jiao, Liu Rongyan, Xu Junyi, Yao Yingjuan, Li Beibei, Chen Dengke, Chang Zhuangpeng, Zhao Rui, Feng Yanlin, Hou Ruigang, Lee Min, Zhang Xiao
Second Clinical Medical College, School of Pharmacy and Key Laboratory of Cellular Physiology, Shanxi Medical University, Taiyuan, Shanxi, 030001, People's Republic of China.
Medicinal Basic Research Innovation Center of Chronic Kidney Disease, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi, 030001, People's Republic of China.
J Nanobiotechnology. 2025 Mar 12;23(1):206. doi: 10.1186/s12951-025-03235-y.
Excessive generation of reactive oxygen species is a hallmark of the osteoporotic bone microenvironment, which leads to the damage of mitochondrial function and the deactivation of osteoblasts. Fruits and vegetables are rich sources of antioxidants, which play a key role in scavenging free radicals and maintaining the body's homeostasis.
Herein, we have developed a type of vesicle coming from carrots as nanoantioxidants to counteract oxidative stress and restore the vitality of osteoblasts for reversing osteoporosis. Nanovesicles are derived from carrot juice using a straightforward extrusion method, resulting in stable membrane structures containing various lipids and homologous active phytochemicals. Nanovesicles can maintain stable structures under normal physiological conditions (pH 7.4) and transform into aggregates in response to the acidic extracellular pH of osteoporosis (pH 4.0). As anticipated, nanovesicles can passively target and aggregate to osteoporotic bone, ease oxidative stress, restore mitochondrial function, promote osteoblastogenesis, and reduce bone loss in osteoporotic mice.
This work presents the first demonstration of nanovesicles derived from carrots as novel nanoantioxidants to realize the long-awaited osteogenesis, contributing to the exploration of a brand-new idea for reversing osteoporosis.
活性氧的过度产生是骨质疏松性骨微环境的一个标志，这会导致线粒体功能受损和成骨细胞失活。水果和蔬菜是抗氧化剂的丰富来源，它们在清除自由基和维持身体内环境稳态方面发挥着关键作用。
在此，我们开发了一种源自胡萝卜的囊泡作为纳米抗氧化剂，以对抗氧化应激并恢复成骨细胞的活力，从而逆转骨质疏松症。纳米囊泡通过简单的挤压方法从胡萝卜汁中获得，形成含有各种脂质和同源活性植物化学物质的稳定膜结构。纳米囊泡在正常生理条件（pH 7.4）下可保持稳定结构，并在骨质疏松症的酸性细胞外pH（pH 4.0）下转变为聚集体。正如预期的那样，纳米囊泡可以被动靶向并聚集到骨质疏松性骨，减轻氧化应激，恢复线粒体功能，促进成骨细胞生成，并减少骨质疏松小鼠的骨质流失。
这项工作首次证明了源自胡萝卜的纳米囊泡作为新型纳米抗氧化剂可实现长期期待的成骨作用，为探索逆转骨质疏松症的全新思路做出了贡献。
