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欧前胡素对玻璃化冷冻小鼠卵母细胞的影响及机制

Effects and Mechanisms of Imperatorin on Vitrified Mouse Oocytes.

作者信息

Feng Yuan, Zhang Mengyuan, Yuan Wenqing, Zhao Dan, Luo Zhixuan, Tang Zihui, Wang Yongheng, Cang Ming

机构信息

The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010030, China.

出版信息

Animals (Basel). 2025 Feb 25;15(5):661. doi: 10.3390/ani15050661.

DOI:10.3390/ani15050661
PMID:40075944
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11898152/
Abstract

Imperatorin (IMP) is a naturally occurring furanocoumarin with beneficial biological activities such as anticancer, antioxidant, and neuromodulatory properties. Currently, the protective effects and mechanisms of IMP on oxidative stress experienced by mouse oocytes after vitrification-thawing remain unclear. To investigate the influence of IMP on mouse oocyte development after vitrification-thawing, we added different concentrations of IMP to the vitrification and thawing media. Results indicated that the addition of 40 μM IMP enhanced post-thaw fertilization capacity, reduced intracellular reactive oxygen species (ROS) levels, and increased intracellular glutathione (GSH) levels. IMP also improved mitochondrial health by alleviating the decrease in mitochondrial membrane potential (MMP) and enhancing mitochondrial distribution. IMP reduced intracellular ROS levels by affecting the transcription of the antioxidant genes , , and and enhancing SOD activity. It also elevated GSH levels via , improved mitochondrial function, and decreased early apoptosis through In conclusion, IMP enhanced ovum health through the alleviation of oxidative stress. The present study provides useful information for further exploration of the molecular mechanisms of IMP in female reproductive cells and offers a novel approach for the improvement of vitrification technology.

摘要

欧前胡素（IMP）是一种天然存在的呋喃香豆素，具有抗癌、抗氧化和神经调节等有益的生物学活性。目前，IMP对玻璃化冷冻-解冻后小鼠卵母细胞氧化应激的保护作用及机制尚不清楚。为了研究IMP对玻璃化冷冻-解冻后小鼠卵母细胞发育的影响，我们在玻璃化冷冻和解冻培养基中添加了不同浓度的IMP。结果表明，添加40μM IMP可提高解冻后的受精能力，降低细胞内活性氧（ROS）水平，并提高细胞内谷胱甘肽（GSH）水平。IMP还通过减轻线粒体膜电位（MMP）的下降和增强线粒体分布来改善线粒体健康。IMP通过影响抗氧化基因、和的转录并增强超氧化物歧化酶（SOD）活性来降低细胞内ROS水平。它还通过提高GSH水平、改善线粒体功能并通过减少早期细胞凋亡。总之，IMP通过减轻氧化应激增强了卵子健康。本研究为进一步探索IMP在雌性生殖细胞中的分子机制提供了有用信息，并为改进玻璃化冷冻技术提供了新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/885a0b4e31bb/animals-15-00661-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/fa9ebb91a229/animals-15-00661-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/c61303702dea/animals-15-00661-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/eb35fd72c0e8/animals-15-00661-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/e3488176438b/animals-15-00661-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/6027cb4d10fb/animals-15-00661-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/a26b370c18be/animals-15-00661-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/885a0b4e31bb/animals-15-00661-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/fa9ebb91a229/animals-15-00661-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/c61303702dea/animals-15-00661-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/eb35fd72c0e8/animals-15-00661-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/e3488176438b/animals-15-00661-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/6027cb4d10fb/animals-15-00661-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/a26b370c18be/animals-15-00661-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f32/11898152/885a0b4e31bb/animals-15-00661-g007.jpg

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Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes.褪黑素对水牛卵母细胞发育能力、线粒体分布和新鲜及玻璃化/解冻体外成熟卵母细胞强度的影响。
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Detrimental effects of vitrification on integrin genes (α9 and β1) and in vitro fertilization in mouse oocytes.
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Mol Biol Rep. 2023 Jun;50(6):4823-4829. doi: 10.1007/s11033-023-08377-6. Epub 2023 Apr 11.
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Imperatorin exerts antioxidant effects in vascular dementia via the Nrf2 signaling pathway.小白菊内酯通过 Nrf2 信号通路在血管性痴呆中发挥抗氧化作用。
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