Reproductive Biology Associates, Atlanta, GA, USA.
Biol Reprod. 2022 Feb 22;106(2):316-327. doi: 10.1093/biolre/ioab239.
Vitrification, is an ultra-rapid, manual cooling process that produces glass-like (ice crystal-free) solidification. Water is prevented from forming intercellular and intracellular ice crystals during cooling as a result of oocyte dehydration and the use of highly concentrated cryoprotectant. Though oocytes can be cryopreserved without ice crystal formation through vitrification, it is still not clear whether the process of vitrification causes any negative impact (temperature change/chilling effect, osmotic stress, cryoprotectant toxicity, and/or phase transitions) on oocyte quality, which translates to diminished embryo developmental potential or subsequent clinical outcomes. In this review, we attempt to assess the technique's potential effects and the consequence of these effects on outcomes.
玻璃化，是一种超快速的、手动冷却的过程，产生类似玻璃的（无冰晶）固化。由于卵母细胞脱水和使用高浓度的冷冻保护剂，在冷却过程中防止水形成细胞内和细胞间冰晶。尽管卵母细胞可以通过玻璃化而不形成冰晶来冷冻保存，但仍不清楚玻璃化过程是否会对卵母细胞质量产生任何负面影响（温度变化/冷休克效应、渗透应激、冷冻保护剂毒性和/或相变），从而降低胚胎发育潜力或后续临床结局。在这篇综述中，我们试图评估该技术的潜在影响及其对结果的影响。
