Gerhard Glenn S, Allard John B, Kaniper Scott, Lynch Dorret, Lee Hayan, Kumar Sudhir
Department of Medical Genetics and Molecular Biochemistry, Lewis Katz School of Medicine at Temple University, Philadelphia, PA 19140, USA.
Institute for Genomics and Evolutionary Medicine, Temple University, Philadelphia, PA 19122, USA.
Animals (Basel). 2025 Feb 27;15(5):690. doi: 10.3390/ani15050690.
We report the first high-quality genome-wide assembly for , the longest-lived non-colonial species, with a reported maximum life span of 507 years. The genome was assembled using short- and long-read DNA sequencing and RNA sequencing of four tissues. All assessment approaches indicated that the assembled genome is complete, contiguous, and accurate. The genome size is estimated at 1781.15 million base pairs (Mbps) with a coverage of 247.8×. The heterozygous rate was 1.15% and the repeat content 67.66%. Genome completeness evaluated by complete BUSCOs was 92.7%. The non-redundant gene set consisted of 39,509 genes with an average transcript length of 15,429 bp. More than 98% of the genes could be annotated across databases. Predicted non-coding RNAs included 801 miRNAs, 11,114 tRNAs, 909 rRNAs, and 349 snRNAs. The genome, along with the assembly of genomes from other clam species, sets the stage for elucidating the molecular basis for the convergence of extreme longevity across these bivalve species.
我们报告了首个针对最长寿的非群居物种（据报道其最大寿命为507年）的高质量全基因组组装结果。该基因组是利用四个组织的短读长和长读长DNA测序以及RNA测序进行组装的。所有评估方法均表明，组装后的基因组完整、连续且准确。基因组大小估计为17.8115亿碱基对（Mbps），覆盖度为247.8倍。杂合率为1.15%，重复序列含量为67.66%。通过完整的BUSCO评估的基因组完整性为92.7%。非冗余基因集由39509个基因组成，平均转录本长度为15429 bp。超过98%的基因可以在多个数据库中进行注释。预测的非编码RNA包括801个miRNA、11114个tRNA、909个rRNA和349个snRNA。该物种的基因组，以及其他蛤类物种的基因组组装，为阐明这些双壳类物种极端长寿趋同的分子基础奠定了基础。
