Ma Peifen, Da Jianlong, Zhao Guanghui, Suo Feiya, Li Yan, Zhou Xiaochun, Li Yao, Han Yiheng, Zou Mingyang, Dou Xinman
The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China.
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Polymers (Basel). 2025 Feb 25;17(5):607. doi: 10.3390/polym17050607.
Diabetic wounds are therapeutically challenging because of the complex and adverse microenvironment that impedes healing. Unlike conventional wound dressings, hydrogels provide antibacterial, anti-inflammatory, and repair-promoting functions. In this study, we developed a light-responsive and injectable chitosan methacryloyl (CSMA) hydrogel, incorporating soy isoflavones (SIs) and gold nanoparticles (AuNPs). Transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and proton nuclear magnetic resonance (1H NMR) spectroscopy analyses confirmed the successful synthesis of the CSMA/SI/AuNP hydrogels. In vitro experiments demonstrated that this hydrogel exhibited exceptional biocompatibility and enhanced the migration of human umbilical vein endothelial cells ( < 0.05), thereby underscoring its potential for promoting angiogenesis. In vivo studies have indicated that hydrogels significantly enhance the rate of wound healing ( < 0.001). Moreover, they facilitate angiogenesis ( < 0.01) and diminish the inflammatory response at the wound site ( < 0.05). Additionally, hydrogels promote collagen deposition and the regeneration of skin appendages. These findings substantiate the hydrogel's therapeutic potential for diabetic wound care, highlighting its promise for regenerative medicine. CSMA/SI/AuNP represents a significant advancement in diabetic wound treatment, addressing key challenges in wound healing by offering a multifaceted therapeutic approach with broad clinical implications for enhancing patient outcomes in chronic wound management.
糖尿病伤口的治疗具有挑战性，因为其复杂且不利的微环境会阻碍伤口愈合。与传统伤口敷料不同，水凝胶具有抗菌、抗炎和促进修复的功能。在本研究中，我们开发了一种光响应性可注射的甲基丙烯酰化壳聚糖（CSMA）水凝胶，其中包含大豆异黄酮（SIs）和金纳米颗粒（AuNPs）。透射电子显微镜（TEM）、傅里叶变换红外（FTIR）光谱和质子核磁共振（1H NMR）光谱分析证实了CSMA/SI/AuNP水凝胶的成功合成。体外实验表明，这种水凝胶具有出色的生物相容性，并能促进人脐静脉内皮细胞的迁移（<0.05），从而突出了其促进血管生成的潜力。体内研究表明，水凝胶能显著提高伤口愈合速度（<0.001）。此外，它们还能促进血管生成（<0.01）并减轻伤口部位的炎症反应（<0.05）。此外，水凝胶还能促进胶原蛋白沉积和皮肤附属器的再生。这些发现证实了水凝胶在糖尿病伤口护理中的治疗潜力，突显了其在再生医学中的前景。CSMA/SI/AuNP代表了糖尿病伤口治疗的重大进展，通过提供一种多方面的治疗方法来应对伤口愈合中的关键挑战，对改善慢性伤口管理中的患者预后具有广泛的临床意义。
