Ríhová B, Ríha I, Rossmann P, Vĕtvicka V
Folia Microbiol (Praha). 1985;30(3):295-301. doi: 10.1007/BF02923523.
The low IgG response of the strain C57BL/10ScSn is not restricted to the reaction to sheep red blood cells; but it can be demonstrated even after immunization with ARS, DNP or FITC haptens, coupled to various heterologous (BGG, RSA) or autologous (MGG) protein carriers. The level of the IgG response is - using the same immunization schedule - influenced both by the bound hapten and the carrier. In both strains tested (i.e. in the high-responding A/J and the low-responding C57BL/10ScSn), the highest IgG response is elicited by FITC-BGG. The response of the C57BL/10ScSn strain is, similarly as after immunization with SRBC, approximately ten times lower. The IgG response to other antigens tested was lower in both strains and therefore the quantitative differences were less pronounced. The affinity of antibodies against the ARS and TNP determinant, detected by inhibition of plaque-forming cells, is similar in the two strains. Thus the low reactivity of the strain C57BL/10ScSn is not caused by the absence of suitable VH and VL genes, but it rather indicates a defect of some general regulatory mechanism, involved in the synthesis of IgG antibodies. After repeated administration of ARS-BGG, the antigen and the antigen - antibody complexes accumulate in high concentrations primarily in the liver of mouse strain A/J. The amount of antigen accumulated in the liver of strain C57BL/10ScSn is significantly lower.
C57BL/10ScSn品系的低IgG反应并不局限于对绵羊红细胞的反应；即使在用与各种异源（BGG、RSA）或同源（MGG）蛋白质载体偶联的ARS、DNP或FITC半抗原免疫后，也能表现出来。使用相同的免疫方案，IgG反应水平受结合的半抗原和载体的影响。在测试的两个品系中（即高反应性的A/J和低反应性的C57BL/10ScSn），FITC-BGG引发的IgG反应最高。C57BL/10ScSn品系的反应与用SRBC免疫后类似，大约低十倍。在两个品系中，对其他测试抗原的IgG反应较低，因此定量差异不太明显。通过抑制空斑形成细胞检测到的针对ARS和TNP决定簇的抗体亲和力在两个品系中相似。因此，C57BL/10ScSn品系的低反应性不是由于缺乏合适的VH和VL基因引起的，而是表明参与IgG抗体合成的一些一般调节机制存在缺陷。重复给予ARS-BGG后，抗原和抗原-抗体复合物主要在A/J小鼠品系的肝脏中高浓度积累。C57BL/10ScSn品系肝脏中积累的抗原量明显较低。
